文献类型：期刊文献

中文题名：用于生物探测的PAMAM树形高分子增强免疫磁性微球

英文题名：PAMAM Dendrimers Enhanced Immunomagnetic Microspheres for Biological Detection

作者：罗吾钧[1];朱以华[1];包华[1];李培勇[2]

机构：[1]华东理工大学超细材料制备与应用教育部重点实验室,上海200237;[2]上海第二医科大学附属瑞金医院核医学科,上海200025

年份：2004

卷号：17

期号：3

起止页码：367

中文期刊名：功能高分子学报

外文期刊名：Journal of Functional Polymers

收录：CSTPCD;;CSCD:【CSCD2011_2012】；

基金：国家自然科学基金(20276019);上海市纳米专项(0359nm001)资助项目.

语种：中文

中文关键词：生物探测;树形高分子;免疫磁性微球;悬浮聚合;聚苯乙烯;牛血清蛋白;共价偶合;单克隆抗体

外文关键词：PAMAM; dendrimer; immunomagnetic microspheres; bovine serum albumin; covalent coupling

摘要：　采用悬浮聚合的方法制备羧基修饰的聚苯乙烯磁性微球(CMM),研究了CMM和CMM PAMAM(第5代)两者与牛血清蛋白(BSA)的结合状况。结果显示:CMM与BSA的结合量为22.2μg/mg。PAMAM偶联在磁性微球表面后,提高了磁性微球表面官能团的密度,因而CMM PAMAM(第5代)与BSA的结合量可达54.4μg/mg。
Carboxyl-modified microspheres (CMM) were synthesized by suspension method. And the content of Bovine serum albumin (BSA) coupled with CMM and CMM-Polyamidoamine (PAMAM) (Generation 5) were investigated. The results show that the content of BSA coupled with CMM is 22.2μg/mg, while the content of BSA coupled with PAMAM-CMM is 54.4μg/mg. This was mainly due to the density of surface functional groups on CMM enhanced greatly after covalent coupled with PAMAM.