文献类型：期刊文献

英文题名：Interactions of the primers and Mg2+ with graphene quantum dots enhance PCR performance

作者：Zhu, Meidong[1];Luo, Chao[1];Zhang, Fangwei[2];Liu, Fei[1];Zhang, Jingyan[1];Guo, Shouwu[2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Elect Informat & Elect Engn, Shanghai 200240, Peoples R China

年份：2015

卷号：5

期号：91

起止页码：74515

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20153701268828);WOS:【SCI-EXPANDED(收录号:WOS:000361116500045)】；

基金：This research was carried out with financial support from the state key laboratory of bioreactor engineering (No. 2060204), 111 Project (No. B07023), the Shanghai Committee of Science and Technology (No. 11DZ2260600), the national "973 Program" of China (No. 2014CB260411, 2015CB931801), National Science foundation of China (No. 11374205), and national "863" Program of China (No. 2012AA022603) for financial support of this work.

语种：英文

外文关键词：Semiconductor quantum dots - Biocompatibility - Medical applications - Metal ions - Nanocrystals - Graphene

摘要：It was reported that graphene oxide (GO), a novel carbon material with unique structural and chemical/ physical properties, could improve the performance of polymerase chain reaction (PCR), similar to many nanomaterials. However, GO, with a large lateral size, exhibits poor biocompatibility, and the mechanism of its effect on PCR remains unclear. Using graphene quantum dots (GQDs) that inherit the basic properties of GO, but with nanometer lateral sizes and much better biocompatibility, we systematically investigated their functional roles in PCR enhancement. The overall performance of PCR in terms of its yield, sensitivity, and specificity can be improved with much less GQDs than of GO. It is found that the stacking of the primers on GQDs improves the sensitivity and specificity of PCR through improving efficiency of base-pairing between the primer and the template. The yield of PCR is improved primarily by GQDs via increasing the activity of polymerase, which is tuned by GQDs through chelating magnesium ions with their peripheral carboxylic groups. The adsorption of the polymerase on GQDs affects marginally the activity of polymerase that is different from the proposed function of GO to PCR. The superb performance of GQDs in PCR exhibits their practical potential in PCR, and thus they can be used in biology and medical applications.