文献类型：期刊文献

英文题名：Adaptive evolution of Paecilomyces variotii enhanced the biodetoxification of high-titer inhibitors in pretreated lignocellulosic feedstock

作者：Chen, Agustian[1];Zhang, Bin[1];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2024

卷号：411

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20243516954245);WOS:【SCI-EXPANDED(收录号:WOS:001306835200001)】；

基金：This research was supported by the National Key R & D Program of China (2023YFA0914400) , the National Natural Science Foundation of China (32301269) , China Postdoctoral Science Foundation (2023M741175) , and the Yangfan Project of Science and Technology Committee of Shanghai Municipality (23YF1409900) .

语种：英文

外文关键词：Paecilomyces variotii; Lignocellulose; Biorefinery; Biodetoxification; Adaptive laboratory evolution; L-lactic acid

摘要：High inhibitor concentrations in lignocellulose feedstock negatively affect the degradation rate of biodetoxification strains. This study designed two adaptive laboratory evolutions in solid substrate and liquid medium to boost the biodetoxification capacity of P. variotii to high titers of lignocellulose-derived inhibitors, resulting in two evolved strains AC70 and ZW70. The results showed that the evolutionary adaptation in liquid medium could better boost the acetic acid assimilation compared to that on solid substrate. Transcriptional analysis revealed that the evolved strains exhibited a significant upregulation of adh, acs, ach1, and ackA directly related to the initial steps of acetate and furan aldehydes metabolisms. ZW70 strain can effectively remove the high concentration inhibitors cocktail from the hydrolysates derived from pretreated wheat straw and furfural residues. The biodetoxified hydrolysates by ZW70 were successfully used for cellulose chiral L-lactic acid production with the titers of similar to 110 g/L, which were over 20 % higher than that detoxified by parental strain.