文献类型：期刊文献

英文题名：A novel impeller configuration to improve fungal physiology performance and energy conservation for cephalosporin C production

作者：Yang, Yiming[1];Xia, Jianye[1];Li, Jianhua[1];Chu, Ju[1];Li, Liang[1];Wang, Yonghong[1];Zhuang, Yingping[1];Zhang, Siliang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：161

期号：3

起止页码：250

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20123515386346);WOS:【SCI-EXPANDED(收录号:WOS:000308354100010)】；

基金：This work was financially supported by a grant from the Major State Basic Research Development Program of China (973 Program), No. 2012CB721006, and Research Fund for the Doctoral Program of Higher Education of China (20110074110015), the National Scientific and Technological Major Special Project (Significant Creation of New drugs), No. 2011ZX09203-001-03, 2011ZX09203-001-19, the National High Technology Research and Development Program of China (863 Program), No. 2006AA020302.

语种：英文

外文关键词：Impeller configuration; Cephalosporin C; Morphology; Computational fluid dynamics

摘要：Effects of impeller configuration on fungal physiology and cephalosporin C production were investigated by an industrial strain Acremonium chrysogenum in a 12 m(3) bioreactor equipped with conventional and novel impeller configuration, respectively. The cell growth and oxygen uptake rate (OUR) profiles were little affected by the impeller configurations. However, differing impeller combinations significantly affected the morphology, which in turn influenced cephalosporin C production. Under the novel impeller configuration, the production of cephalosporin C was 10% higher and an excessive amount of dispersed arthrospores was also observed. Computational fluid dynamics (CFD) simulation further revealed that poor mass and energy exchange as well as inhomogeneous environment existed in the bioreactor equipped with conventional impeller configuration. For equivalent power dissipation, the volume oxygen transfer coefficient (K(L)a) could be enhanced by 15% compared with that of conventional impeller configuration. Power consumption was dramatically decreased by 25% by using novel impeller configuration. (C) 2012 Elsevier B.V. All rights reserved.