文献类型：期刊文献

英文题名：Pathway engineering and medium optimization for α-farnesene biosynthesis in oleaginous yeast Yarrowia lipolytica

作者：Liu, Shun-Cheng[1];Liu, Zhijie[2];Wei, Liu-Jing[1];Hua, Qiang[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Hubei Univ Technol, Hubei Collaborat Innovat Ctr Ind Fermentat, Hubei Key Lab Ind Microbiol, Minist Educ,Key Lab Fermentat Engn, Wuhan 430068, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：319

起止页码：74

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20202508844657);WOS:【SCI-EXPANDED(收录号:WOS:000542948300009)】；

基金：This work was supported by National Key R&D Program of China (2019YFA0904302), National Natural Science Foundation of China (21776081) and the 111 Project (B18022).

语种：英文

外文关键词：?-farnesene; acetyl-CoA; Metabolic engineering; MVA pathway; Medium optimization

摘要：Farnesene is a typical sesquiterpene with applications as fragrance, flavor and precursor for the synthesis of vitamin E/K1. In this study, a series of strategies were employed to facilitate ?-farnesene accumulation in Yarrowia lipolytica . Among them, the promoter optimization of OptFSLERG20 , Sc-tHMG1 and IDI resulted in more than 62 % increase in ?-farnesene production. Together with the overexpression of Yl-HMGR and ERG19 , ?- farnesene content was signi ficantly improved by more than 3.5 times. The best metabolic engineered strain obtained was therefore used for a uniform design in shake flasks to determine the optimal medium compositions. Furthermore, a maximum ?-farnesene production of approximately 2.57 g/L (34 mg/g DCW) was obtained in fed -batch fermentation where glycerol was supplemented as the feeding carbon source when initial glucose was depleted. This study has laid a good foundation for the development of Y. lipolytica as a promising chassis microbial cell for heterologous biosynthesis of ?-farnesene and other sesquiterpenes.