文献类型：期刊文献

英文题名：Tuning the bioactivity of bone morphogenetic protein-2 with surface immobilization strategies

作者：Chen, Rui[1,3];Yu, Yuanman[1,3];Zhang, Wenjing[1,3];Pan, Yuanzhong[1,3];Wang, Jing[1,3];Xiao, Yin[4];Liu, Changsheng[1,2,3]

机构：[1]East China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China;[4]Queensland Univ Technol, Australia China Ctr Tissue Engn & Regenerat Med, Brisbane, Qld 4059, Australia

年份：2018

卷号：80

起止页码：108

外文期刊名：ACTA BIOMATERIALIA

收录：;EI(收录号：20215111341363);WOS:【SCI-EXPANDED(收录号:WOS:000449133400008)】；

基金：The authors wish to express their gratitude to the financial supports from the National Natural Science Foundation of China (No. 31330028, No. 31470923), the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), International Cooperation Project of Shanghai Science and Technique Committee (15520711100), and Key Project of Logistic Scientific Research of PLA (BWS13C014). This study is also supported by 111 Project (B14018). Shanghai Synchrotron Radiation Facility is also gratefully acknowledged.

语种：英文

外文关键词：BMP-2; Osteogenic activity; Immobilization; Heparin; Bone regeneration

摘要：Bone morphogenetic protein-2 (BMP-2) involved therapy is of great potential for bone regeneration. However, its clinical application is restricted due to the undesirable bioactivity and relevant complications in vivo. Immobilization of recombinant BMP-2 (rhBMP-2) is an efficient strategy to mimic natural microenvironment and retain its bioactivity. Herein, we present evidences indicating that osteoinductive capacity of rhBMP-2 can be regulated via variant immobilizing approaches. Three representative superficial immobilizing models were employed to fabricate rhBMP-2-immobilized surfaces including physical adsorption (Au/rhBMP-2), covalent grafting (rhBMP-2-SAM-Au) and heparin binding (Hep-SAM-Au/rhBMP-2) (SAM: self-assembled monolayer). Loading capacity, releasing behavior, osteogenic differentiation and signaling pathways involved, as well as the cellular recognition of rhBMP-2 under various immobilization modes were systematically investigated. As a result, disparate immobilizing approaches not only have effects on loading capacity, but also lead to disparity of osteoinduction at the same dosage. Notably, heparin could reinforce the recognition between rhBMP-2 and its receptors (BMPRs) whereas weaken its binding to its antagonist Noggin. Owing to this "selective" binding feature, the favorable osteoinduction and maximum ectopic bone formation can be achieved with the heparin-binding approach. In particular, manipulation of orientation-mediated BMP-2-cell recognition efficiency may be a potential target to design more therapeutic efficient rhBMP-2 delivery system. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.