文献类型：期刊文献

英文题名：Effect of the culture media optimization, pH and temperature on the biohydrogen production and the hydrogenase activities by Klebsiella pneumoniae ECU-15

作者：Xiao, Yan[1];Zhang, Xu[1];Zhu, Minglong[1];Tan, Wensong[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：137

起止页码：9

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20131716231417);WOS:【SCI-EXPANDED(收录号:WOS:000319645600002)】；

基金：The author was grateful for the financial support of this research by the National Special Fund of China for State Key Laboratory of Bioreactor Engineering, Grant No. 2060204.

语种：英文

外文关键词：Biohydrogen production; Response surface methodology (RSM); Hydrogenase; Medium compositions; Klebsiella pneumoniae

摘要：The low yield of the biohydrogen production is the main constraint for its industrialization process. In order to improve its production, medium compositions of the hydrogen fermentation by Klebsiella pneumoniae ECU-15 were optimized through the response surface methodology (RSM). Experimental results showed that the optimum hydrogen production of 5363.8 ml/L was obtained when the concentration of glucose, the ammonium sulfate and the trace elements were 35.62 g/L, 2.78 g/L and 23.15 ml/L at temperature 37.0 degrees C, pH 6.0. H-2 evolving hydrogenase was greatly enhanced by the optimization of the medium compositions. The activity of H-2 evolving hydrogenase increased with the temperature, and decreased with the pH, while the activity of the uptake hydrogenase increased with the temperature and the pH. So the biohydrogen production process of the K. pneumoniae ECU-15 was the comprehensive results of the evolution hydrogen process and the uptake hydrogen process. (C) 2013 Elsevier Ltd. All rights reserved.