文献类型：期刊文献

英文题名：Selection and screening strategies in directed evolution to improve protein stability

作者：Ren, Chang[1];Wen, Xin[1];Mencius, Jun[1];Quan, Shu[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Collaborat Innovat Ctr Biomfg, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：6

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513084729);WOS:【SCI-EXPANDED(收录号:WOS:000511727600001)】；

基金：This work was supported by the National Natural Science Foundation of China (NSFC) (Grant Nos. 31661143021, 31670802, and 31870054 to S. Quan), the Fundamental Research Funds for the Central Universities (Grant No. 22221818014), and the Research Program of State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Directed evolution; Protein folding; Protein stability; Cell surface display; Cell-free display; Reporter protein; Stability biosensor

摘要：Protein stability is not only fundamental for experimental, industrial, and therapeutic applications, but is also the baseline for evolving novel protein functions. For decades, stability engineering armed with directed evolution has continued its rapid development and inevitably poses challenges. Generally, in directed evolution, establishing a reliable link between a genotype and any interpretable phenotype is more challenging than diversifying genetic libraries. Consequently, we set forth in a small picture to emphasize the screening or selection techniques in protein stability-directed evolution to secure the link. For a more systematic review, two main branches of these techniques, namely cellular or cell-free display and stability biosensors, are expounded with informative examples.