文献类型：期刊文献

英文题名：Controlled synthesis of PEGylated polyelectrolyte nanogels as efficient protein carriers

作者：Zhou, Lu[1];Gao, Yifan[1];Cai, Ying[1];Zhou, Jin[1];Ding, Peng[1];Stuart, Martien A. Cohen[1];Wang, Junyou[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Multiphase Mat Chem Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：620

起止页码：322

外文期刊名：JOURNAL OF COLLOID AND INTERFACE SCIENCE

收录：;EI(收录号：20221611965982);WOS:【SCI-EXPANDED(收录号:WOS:000793567600005)】；

基金：This work was financially supported by 1000 Foreign Experts Program (WQ20163100341), the Shanghai Municipal Natural Science Foundation (17ZR1440500, 18490740100) and the Fundamental Research Funds for the Central Universities (222201714003).

语种：英文

外文关键词：Polyelectrolyte; Nanogels; PEGylation; Protein encapsulation; Protection and delivery

摘要：Designing rational carriers for protein encapsulation and delivery is of great interest in a wide range of applications. Herein, we develop a type of PEGylated polyelectrolyte nanogel that enables efficient pro-tein loading, protection and intracellular delivery. Our design relies on a one pot process involving coop-erative electrostatic assembly and polymerization, which constructs polyion complex (PIC) micelles containing cross-linked cationic PDMAEMA network associated with anionic PAA. Further removing PAA chains leads to core-shell nanogels with a cationic network surrounded by poly (ethylene glycol) (PEG) blocks. The established strategy allows to fabricate well-defined nanogels with regulated size and swelling ability depending on pH, salt concentration and cross-link degree. More notably, the abun-dant positive charges and the thermo-response of PDMAEMA network realize sufficient encapsulation and protection of BSA proteins, while the PEG blocks endow appreciable biocompatibility and successful intracellular protein delivery. Our study establishes a facile and robust approach for preparing core-shell polyelectrolyte nanogels with regulated size and properties, as well as prominent capabilities for protein encapsulation and delivery.(c) 2022 Elsevier Inc. All rights reserved.