文献类型：期刊文献

英文题名：Calcium Carbonate/Gelatin Methacrylate Microspheres for 3D Cell Culture in Bone Tissue Engineering

作者：Xu, Pengwei[1];Jiang, Fuliang[1];Zhang, Hongbo[1];Yin, Ruixue[1];Cen, Lian[2];Zhang, Wenjun[3,4]

机构：[1]East China Univ Sci & Technol, Sch Mech & Power Engn, 130 Meilong Rd, Shanghai 200237, Xuhui District, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem Engn, Shanghai, Peoples R China;[3]Shanghai Univ, Sch Mechatron & Automat, 99 ShangDa Rd, Shanghai 200444, Baoshan Distric, Peoples R China;[4]Univ Saskatchewan, Div Biomed Engn, Saskatoon, SK, Canada

年份：2020

卷号：26

期号：8

起止页码：418

外文期刊名：TISSUE ENGINEERING PART C-METHODS

收录：;EI(收录号：20203709152178);WOS:【SCI-EXPANDED(收录号:WOS:000556956400001)】；

基金：This work is financially supported by the Shanghai Science and Technology Innovation Program (No. 19441909600) and the Opening project of Shanghai Key Laboratory of Orthopedic Implant (KFKT2020001).

语种：英文

外文关键词：microfluidics; GelMA; CaCO(3)microsphere; cell adhesion; osteogenesis; bone tissue engineering

摘要：Impact statement Microspheres as cell culture substrates have attracted a great deal of attention. The combination of organic and inorganic materials offers the unique merits in bone tissue engineering. In this study, there are two contributions. First, the organic and inorganic material of gelatin methacrylate (GelMA) and CaCO(3)were successfully combined, especially, CaCO(3)was formed as crystals to enhance cell attachment. Second, microspheres were successfully fabricated with one-step process: that is, the microfluidic technique was coupled with the CaCO(3)precipitationin situ. Cell culture shows that the GelMA/CaCO(3)microspheres proposed in this study have a high potential for bone tissue engineering applications. Hydrogel microspheres have been widely used as cell carriers and three-dimensional cell culture matrices. However, these microspheres are associated with several unfavorable properties for bone tissue engineering applications, for example, their surface is too smooth to attach cells and they do not contain inorganic materials. This article presents a new method to overcome these disadvantages by depositing CaCO(3)crystals on the hydrogel microsphere surface. Specifically, we used a nonplanar flow-focusing microfluidic device to produce gelatin methacrylate (GelMA)-/Na2CO3-based microspheres. We subsequently obtained CaCO(3)crystals by a chemical reaction between Na(2)CO(3)and CaCl2. The efficacy of this method was demonstrated byin vitroexperiments with human umbilical vein endothelial cells (HUVEC) and immortalized mouse embryonic fibroblasts (iMEF). Cell culture on GelMA/CaCO(3)microspheres showed that cells can easily attach and adhere to GelMA/CaCO(3)microspheres and maintain high viability. Alkaline phosphatase (ALP) expression was increased as well. These results suggest that this novel microsphere has a high potential for bone tissue engineering applications.