文献类型：期刊文献

英文题名：Polyketides in Aspergillus terreus: biosynthesis pathway discovery and application

作者：Yin, Ying[1];Cai, Menghao[1];Zhou, Xiangshan[1];Li, Zhiyong[2];Zhang, Yuanxing[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Marine Biotechnol Lab, State Key Lab Microbial Metab, 800 Dongchuan Rd, Shanghai 200240, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：100

期号：18

起止页码：7787

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20163102654348);WOS:【SCI-EXPANDED(收录号:WOS:000382008000001)】；

基金：This study was funded by the National High Technology Research and Development Program of China (2011AA090702).

语种：英文

外文关键词：Polyketide; Aspergillus terreus; Biosynthesis pathway; Gene cluster; Heterologous expression

摘要：The knowledge of biosynthesis gene clusters, production improving methods, and bioactivity mechanisms is very important for the development of filamentous fungi metabolites. Metabolic engineering and heterologous expression methods can be applied to improve desired metabolite production, when their biosynthesis pathways have been revealed. And, stable supplement is a necessary basis of bioactivity mechanism discovery and following clinical trial. Aspergillus terreus is an outstanding producer of many bioactive agents, and a large part of them are polyketides. In this review, we took polyketides from A. terreus as examples, focusing on 13 polyketide synthase (PKS) genes in A. terreus NIH 2624 genome. The biosynthesis pathways of nine PKS genes have been reported, and their downstream metabolites are lovastatin, terreic acid, terrein, geodin, terretonin, citreoviridin, and asperfuranone, respectively. Among them, lovastatin is a well-known hypolipidemic agent. Terreic acid, terrein, citreoviridin, and asperfuranone show good bioactivities, especially anticancer activities. On the other hand, geodin and terretonin are mycotoxins. So, biosynthesis gene cluster information is important for the production or elimination of them. We also predicted three possible gene clusters that contain four PKS genes by homologous gene alignment with other Aspergillus strains. We think that this is an effective way to mine secondary metabolic gene clusters.