文献类型：期刊文献

英文题名：A Newly Isolated Penicillium oxalicum 16 Cellulase with High Efficient Synergism and High Tolerance of Monosaccharide

作者：Zhao, Xi-hua[1];Wang, Wei[2];Tong, Bin[1];Zhang, Su-ping[3];Wei, Dong-zhi[2]

机构：[1]Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Peoples R China;[2]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Res Ctr Biomass Energy, Shanghai 200237, Peoples R China

年份：2016

卷号：178

期号：1

起止页码：173

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20154301438442);WOS:【SCI-EXPANDED(收录号:WOS:000368686200013)】；

基金：This work was supported by the National Basic Research Program of China (973, Program NO. 2012CB721103), National High Technology Research and Development Program of China (863, Program NO. 2012AA101806), National Natural Science Foundation of China (31360217), and Doctoral Starting up Foundation of Jiangxi Normal University (5451).

语种：英文

外文关键词：Penicillium oxalicum 16; Cellulase; Glucose yield; Synergism; Monosaccharide inhibition

摘要：Compared to Trichoderma reesei RUT-C30 cellulase (Trcel), Penicillium oxalicum 16 cellulase (P16cel) from the fermentation supernatant produced a 2-fold higher glucose yield when degrading microcrystalline cellulose (MCC), possessed a 10-fold higher beta-glucosidase (BGL) activity, but obtained somewhat lower other cellulase component activities. The optimal temperature and pH of beta-1,4-endoglucanase, cellobiohydrolase, and filter paperase from P16cel were 50-60 A degrees C and 4-5, respectively, but those of BGL reached 70 A degrees C and 5. The cellulase cocktail of P16cel and Trcel had a high synergism when solubilizing MCC and generated 1.7-fold and 6.2-fold higher glucose yields than P16cel and Trcel at the same filter paperase loading, respectively. Additional low concentration of fructose enhanced the glucose yield during enzymatic hydrolysis of MCC; however, additional high concentration of monosaccharide (especially glucose) reduced cellulase activities and gave a stronger monosaccharide inhibition on Trcel. These results indicate that P16cel is a more excellent cellulase than Trcel.