文献类型：期刊文献

英文题名：Periparturient transmission of Cryptosporidium xiaoi from ewes to lambs

作者：Ye, Jianbin[1,2];Xiao, Lihua[2];Wang, Yuanfei[1];Wang, Lin[1];Amer, Said[2,3];Roellig, Dawn M.[2];Guo, Yaqiong[1,2];Feng, Yaoyu[1]

机构：[1]E China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA;[3]Kafr El Sheikh Univ, Fac Sci, Dept Zool, Kafe El Sheikh 33516, Egypt

年份：2013

卷号：197

期号：3-4

起止页码：627

外文期刊名：VETERINARY PARASITOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000328010500028)】；

基金：This work was supported by the National Natural Science Foundation of China (31229005, 31110103901); Fundamental Research Funds for the Central Universities, China; and the National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204); and Centers for Disease Control and Prevention.

语种：英文

外文关键词：Cryptosporidium; Periparturient rise; Sheep; Genotyping; Epidemiology

摘要：The mechanism for the maintenance of Cryptosporidium infection in sheep between yearly lambing periods is not clear. Previously, periparturient shedding of oocysts as the result of stress from lambing was suspected to be a mechanism for the initiation of Cryptosporidium infection in lambs, but this has never been verified by genotyping studies. In this study, fecal specimens from four age groups of sheep in Inner Mongolia, China were examined for Cryptosporidium spp. by PCR-restricted fragment length polymorphism and sequence analyses of the small subunit (SSU) rRNA gene, including 59 ewes 1 week before parturition, 154 ewes at parturition, 87 lambs of 3-4 weeks, and 75 lambs of 15-16 weeks. The Cryptosporidium infection rate in ewes at parturition (7.8%) was significantly higher than at 1 week before parturition (1.7%). Higher infection rates were found in lambs (18.4% and 26.7% for 3-4-week-old and 15-16-week-old lambs, respectively). Most (10/13)Cryptosporidium-positive ewes were shedding Cryptosporidium xiaoi, which was also the dominant species (15/16) in neonatal lambs of 3-4 weeks in age (15/16). The less common species in ewes, Cryptosporidium ubiquitum, was not found in lambs of 3-4 weeks but was the dominant species (14/20) in lambs of 15-16 weeks. The major zoonotic Cryptosporidium species, C. parvum (of the IIaA15G2R1 subtype), was only found in one lamb. These data support the occurrence of periparturient transmission of Cryptosporidium spp., especially C xiaoi, from ewes to lambs. Published by Elsevier B.V.