文献类型：期刊文献

英文题名：Development of reaction-free and mitochondrion-immobilized fluorescent probe for monitoring pH change

作者：Guo, Lifang[1,2];Liu, Hao[1,2];Jin, Xin[1,2];Zhang, Zhiyun[1,2];Su, Jianhua[1,2];Yu, Xiaoqiang[3,4]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Joint Int Res Lab Precis Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Shandong Univ, Ctr Bio & Micro Nano Funct Mat, State Key Lab Crystal Mat, Jinan 250100, Peoples R China;[4]Shandong Univ, Adv Med Res Inst, Jinan 250100, Peoples R China

年份：2021

卷号：341

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20211810277535);WOS:【SCI-EXPANDED(收录号:WOS:000652631600006)】；

基金：We thank the financial support from the NSFC/China (Nos. 21788102, 21905090, 21790361, 22004036, 51773111, and 52073163) , Shanghai Municipal Science and Technology Major Project (No. 2018SHZDZX03) , the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning, Programme of Introducing Talents of Discipline to Universities (No. B16017) , Shanghai Science and Technology Committee (No. 17520750100) , Natural Science Foundation of Shanghai (19ZR1412200) , and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Fluorescent probe; Mitochondrial pH; Reaction-free; Mitochondrion-immobilized way

摘要：Mitochondrial pH is an indispensable parameter of reflecting mitochondrial function, and its abnormity is closely associated with various diseases, such as cardiovascular diseases, Alzheimer's disease, and Reye's syndrome. For real-time and long-term monitoring of mitochondrial pH changes, we for the first time developed a free-reaction and mitochondrion-immobilized pH-sensitive fluorescent probe (MTpH). This probe consists of a cationic pyridinium for targeting mitochondrion, a long alkyl chain for fixing on the mitochondrion, and a morpholine-linked naphthalimide as a fluorescence turn-on signaling unit to pH change. By linking a long alkyl chain, probe MTpH could successfully immobilize on the mitochondrion in a reaction-free way, which efficiently avoided the irreversible destroy to the original structure and function of the mitochondrion. Importantly, this probe also showed a sensitive response to pH changes with the pKa of 5.77, allowing for monitoring mitochondrial pH changes in impaired mitochondria undergoing mitophagy. Therefore, the strategy in this context provides a new thought for designing mitochondrion-immobilized pH-sensitive probes and our probe MTpH is promising as a useful tool for long-term monitoring of mitochondrial pH changes.