文献类型：期刊文献

英文题名：Immune responses of zebrafish (Danio rerio) induced by bath-vaccination with a live attenuated Vibrio anguillarum vaccine candidate

作者：Zhang, Zhihui[1];Wu, Haizhen[1];Xiao, Jingfan[1];Wang, Qiyao[1];Liu, Qin[1];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：33

期号：1

起止页码：36

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000305378400005)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (No. 31001121), the Fundamental Research Funds for the Central Universities (No. WF0914039), Program for New Century Excellent Talents in University (No. Ncet-09-0344) and the Shanghai Leading Academic Discipline Project 278 (No. B505).

语种：英文

外文关键词：Bath-vaccination; Danio rerio; Immunoprotection; Live attenuated vaccine; Vibrio anguillarum

摘要：A fish vaccine candidate, live attenuated Vibrio anguillarum, which can protect fish from vibriosis, was established in our laboratory. In this study, the protective immunological mechanism of live attenuated V. anguillarum was investigated in zebrafish as a model animal. After bath-vaccinated with the live attenuated strain, zebrafish were challenged with wild pathogenic strain to test the immunoprotection of the live attenuated strain. As the results, specific antibody response of fish against V. anguillarum was found to gradually increase during 28 days post-vaccination, and remarkable protection was showed with a high relative protection survival (RPS) of about 90%. Moreover, the vaccination changed the expressions of several immune-related genes in the spleens and livers of zebrafish. Among them, the expressions of pro-inflammatory factors such as IL-1 and IL-8 were tenderly up-regulated with about 3-4 fold in 1-7 days post-vaccination, while MHC II rose to a peak level of 4-fold in 7th day post-vaccination. These results gave some important messages about the mechanism of specific protection induced by live attenuated V. anguillarum and showed the availability of zebrafish model in the evaluation of the vaccine candidate. (C) 2012 Elsevier Ltd. All rights reserved.