文献类型：期刊文献

英文题名：Isolation and Antibiotic Screening of Fungi from a Hydrothermal Vent Site and Characterization of Secondary Metabolites from a Penicillium Isolate

作者：Pan, Chengqian[1];Shi, Yutong[1];Auckloo, Bibi Nazia[1];ul Hassan, Syed Shams[1];Akhter, Najeeb[1];Wang, Kuiwu[2];Ye, Ying[1];Chen, Chen-Tung Arthur[3];Tao, Xinyi[4];Wu, Bin[1]

机构：[1]Zhejiang Univ, Ocean Coll, Hangzhou 310058, Zhejiang, Peoples R China;[2]Zhejiang Gongshang Univ, Dept Appl Chem, Hangzhou 310018, Peoples R China;[3]Natl Sun Yat Sen Univ, Inst Marine Geol & Chem, Kaohsiung 80424, Taiwan;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：19

期号：5

起止页码：469

外文期刊名：MARINE BIOTECHNOLOGY

收录：;EI(收录号：20172903942951);WOS:【SCI-EXPANDED(收录号:WOS:000410777100005)】；

基金：This work was supported by NSFC (Nos. 81273386 and 81573306). We thank Prof. Hu-Jun Xie from the School of Food Science and Biotechnology, Zhejiang Gongshang University, for his kind help on the calculation of the ECD spectra and determination of the configurations.

语种：英文

外文关键词：Marine fungi; Isocoumarin; Antibacterial; New skeleton

摘要：Five new compounds were isolated from Penicillium sp. Y-5-2 including an austin derivative 4, four isocoumarins 9, 11, 12, and 13, together with two known isocoumarins 8 and 10, and six known austin derivatives 1, 2, 3, 5, 6, and 7 and one phenol 14. Their structures and relative configurations were established by spectroscopic means. The absolute configurations of 4, 11, and 13 were defined mainly by comparison of quantum chemical TDDFT calculated and experimental ECD spectra. The cyclization of the pentan-2-ol pendant at C-3 in compound 13 allowed the assignment of a new 2,3,4,4a,6,10b-hexahydro-1H-benzo[c]chromene isocoumarin skeleton. New compounds 9, 11, and 13 revealed inhibitory activities against E. coli at MIC values around 32 mu g/mL. The known compound 14 showed potent antibiotic activity against Staphylococcus aureus and Bacillus subtilis with MIC values 8 and 2 mu g/mL, respectively, with no cytotoxicity when tested in vitro. A rapid and efficient technique for selecting antibiotic fungal strain among eight marine-derived fungi was also described.