文献类型：期刊文献

英文题名：Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification

作者：Tian, Xuena[1,2];Jin, Jiayang[1,2];Zhu, Xuan[1,2];Wang, Yibing[1,2];Qiu, Yongjun[1,2];Qiao, Liangzhi[1,2];Zhao, Liming[1,2]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2026

卷号：1784

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20262721033097);WOS:【SCI-EXPANDED(收录号:WOS:001818157000001)】；

基金：This work was supported by the Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China (JYB2025XDXM509) .

语种：英文

外文关键词：Mrna capture; Oligo(dT) chromatography; Affinity chromatography; Chromatography resins

摘要：With the rapid development of the mRNA pharmaceutical industry, Oligo(dT) chromatography, as a core step in mRNA separation and purification, has garnered increasing attention from both the industry and researchers. This article begins with the fundamental principles of mRNA affinity chromatography, analyzes the advantages and disadvantages of two typical affinity techniques, and highlights the sufficiency of Oligo(dT) chromatography as a platform technology. Aiming to the poor performance of the Oligo(dT) chromatography, the article reviews the main methods and mechanisms for enhancing its performance from three perspectives, resin structure, ligand structure, and process intensification. It also highlights the factors influencing the performance of Oligo(dT) chromatography and the challenges associated with these methods. The article provides an outlook on the future development directions of Oligo(dT) chromatography, offering insights for the advancement of next-generation Oligo(dT) chromatography resins and processes.