文献类型：期刊文献

英文题名：Revealing TCA Cycle Flow Dynamics in Fungicide-Adapted Botrytis cinerea through Anionic Chromatographic Simultaneous Separation

作者：Zhao, Yanjun[1];Zhao, Sijia[2];Wang, Xiaowei[2];Luan, Shaorong[2];Xiao, Ciying[3];Huang, Qingchun[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China

年份：2025

卷号：73

期号：22

起止页码：14079

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20252118464461);WOS:【SCI-EXPANDED(收录号:WOS:001492372500001)】；

基金：This work was financially supported by the National Natural Science Foundation (32372588) and the projects of the Science and Technology Plan of Shanghai Municipality in China (22N41900100 and 21S11901400).

语种：英文

外文关键词：TCA cycle flow; simultaneous separation; fungicideadaptation; Botrytis cinerea

摘要：The exploration of new strategies to monitor Botrytis cinerea populations is critical for disease management. This study achieved efficient and simultaneous separation of all TCA cycle intermediates in mycelia treated with pyraclostrobin (PYR), tebuconazole (TEB), and carbendazim (CAR) using anionic chromatography. Resolutions of nine organic acids and six inorganic anions, including all TCA cycle intermediates, exceeded 1.5. The fungicides inhibited mycelial growth concentration-dependently, but prolonged exposure reduced the level of inhibition. TEB decreased fumarate and malate levels at 48 h, increased citrate, isocitrate, and oxalate levels and reduced alpha-ketoglutarate levels at 96 h. PYR showed biphasic effects, elevating fumarate, malate, and succinate at both time points but reducing citrate and succinate at 96 h. CAR attenuated TCA cycle flow, with isocitrate and alpha-ketoglutarate declining continuously. Moreover, TEB promoted oxalate accumulation, PYR had no effect, and CAR suppressed it. These findings enable precise TCA cycle assessment for fungicide adaption in B. cinerea, aiding Botrytis management.