文献类型：期刊文献

中文题名：腰痛宁胶囊组方不同有效部位细胞药理活性评价

英文题名：Pharmacological Evaluation on Different Active Fractions of Yao-Tong-Ning Capsule Based on Cell Models

作者：倪力军[1];朱婷婷[1];王南南[1];张立国[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237

年份：2015

卷号：17

期号：5

起止页码：963

中文期刊名：世界科学技术-中医药现代化

外文期刊名：Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

收录：CSTPCD

基金：上海市科委支撑计划项目(13401901100):治疗风湿痹病中药有效部位的组合化系统筛选与成药性研究;负责人:倪力军

语种：中文

中文关键词：腰痛宁胶囊;有效部位;免疫调节;抗炎;骨关节炎

外文关键词：Yao-Tong-Ning Capsule; active fractions; immune regulation; anti-inflammation; osteoarthritis

摘要：目的:评价腰痛宁胶囊组方、拆方及其有效部位对细胞增殖以及免疫、抗炎和骨细胞修复功能的影响。方法:按照腰痛宁胶囊组方原则及组合化学思想,根据腰痛宁组方药材比例配制了由腰痛宁不同类型有效部位组成的6个腰痛宁组方及拆方样品,采用半数有效浓度(EC50)或半数抑制浓度(IC50)评价各样品对巨噬(Ana-1)细胞分泌IL-1β、IL-6、TNF-α炎症因子的促进作用;对脂多糖(LPS)诱导的Ana-1细胞释放前列腺素E2(PGE2)产生的抑制作用;对IL-1β诱导的软骨细胞增殖及葡萄糖胺聚糖蛋白(GAG)合成的影响以及对IL-1β诱导的滑膜细胞增殖及IL-6分泌水平的影响,通过比较各样品的EC50(或IC50)叠加值与实验值间的差异分析各有效部位间的相互作用。结果:同一药理模型下6个样品的活性存在差异,部分腰痛宁拆方的某些药理活性优于或显著优于腰痛宁全方,但腰痛宁全方在促进细胞免疫、抗炎及骨细胞修复方面均有良好的功效,腰痛宁药引黄酒有促进各有效部位协同增效的作用。结论:腰痛宁胶囊组方中各有效部位间的协同增效作用是腰痛宁全方具有增强免疫、抗炎及促进骨细胞修复等综合疗效的物质基础。
This study was aimed to evaluate the effects of Yao-Tong-Ning Capsule（YTNC） whole formula, dissembled YTNC formulas, and their active fractions on cell proliferation, immunity, anti-inflammation and osteocytes repair. According to the formulating principle of YTNC and combinatorial chemistry concepts, six samples were prepared by combining different active fractions. Half-maximal effective concentrations（EC50） or half-maximal inhibitory concentrations（IC50） were applied to evaluate the effects of samples on promoting the secretion of IL-1β, IL-6 and TNF-α in macrophage（Ana-1） cells, on inhibiting the production of lipopolysaccharide（LPS）-induced PGE2 in Ana-1 cells, on promoting the cell proliferation induced by IL-1β and glycosaminoglycan（GAG） synthesis, on influencing synoviocytes proliferation induced by IL-1β, and on promoting the secretion of IL-6, respectively. The interactions among the active fractions in these samples were investigated by comparing the additive EC50（or IC50） values with their experimental EC50（or IC50）. The results showed that pharmacological activities of the six samples were different in the same cell model. Some pharmacological activities of a few dissembled YTNC formulas were superior or significantly superior to the YTNC whole formula. However, the YTNC whole formula was good at promoting cell immunity, anti-inflammation and osteocytes repair. The YTNC＇s vehicle Chinese rice wine played an important role on strengthening the activity of YTNC. It was concluded that the synergistic effects between active fractions in YTNC were the material foundation that YTNC had comprehensive efficacy of strengthening immunity, anti-inflammation and promoting osteocytes repair.