文献类型：期刊文献

英文题名：An engineered Pseudomonas putida can simultaneously degrade organophosphates, pyrethroids and carbamates

作者：Gong, Ting[1];Xu, Xiaoqing[1];Dang, Yulei[1];Kong, Annie[1];Wu, Yunbo[1];Liang, Peixin[1];Wang, Shufang[2];Yu, Huilei[3];Xu, Ping[4];Yang, Chao[1]

机构：[1]Nankai Univ, Minist Educ, Key Lab Mol Microbiol & Technol, Tianjin 300071, Peoples R China;[2]Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China

年份：2018

卷号：628-629

起止页码：1258

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20180804824399);WOS:【SCI-EXPANDED(收录号:WOS:000432462000124)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 31570035), the National Key Technology Support Program of China (No. 2015BAD16B04), the Open Funding of State Key Laboratory of Microbial Technology, Shandong University (No. M2016-03), and the Open Funding of State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University (No. MMLKF16-02).

语种：英文

外文关键词：Pesticide; Bioremediation; Metabolic engineering; Pseudomonas putida KT2440

摘要：Agricultural soils are often polluted with a variety of pesticides. Unfortunately, natural microorganisms lack the capacity to simultaneously degrade different types of pesticides. Currently, synthetic biology provides powerful approaches to create versatile degraders. In this work, a biosafety strain Pseudomonas putida KT2440 was engineered for simultaneous degradation of organophosphates, pyrethroids, and carbamates, enhanced oxygen-sequestering capability, and real-time monitoring by targeted insertion of four pesticide-degrading genes, vgb, and gfp into the chromosome using a starless genome-editing method. The resulting recombinant strain, designated as P. putida KTUe, could completely degrade 50 mg/L methyl parathion, chlorpyrifos, fenpropathrin, cypermethrin, carbofuran and carbaryl within 30 h when incubated in M9 minimal medium supplemented with 20 g/L glucose. In soil remediation studies, all the tested six pesticides (50 mg/kg soil each) were completely removed in soils inoculated with P. putida KTUe within 15 days. Moreover, Vitreoscilla hemoglobin (VHb) expressing P. putida KTUe grew faster than P. putida KTUd without VHb expression under oxygen limited conditions, suggesting that VHb may enhance the capability of this recombinant strain to sequester oxygen. Furthermore, the green fluorescence was observed on the P. putida KTUe cells, suggesting that this green fluorescent protein (GFP)-marked strain may be tracked by fluorescence during bioremediation. Therefore, this recombinant strain may serve as a promising candidate for in situ bioremediation of soil contaminated with multiple pesticides. This work not only underscores the value of P. putida KT2440 as an ideal host for bioremediation but also highlights the power of synthetic biology for expanding the degradation capability of natural degraders. (C) 2018 Elsevier B.V. All rights reserved.