文献类型：期刊文献

英文题名：Characterization of the bagremycin biosynthetic gene cluster in Streptomyces sp. Tü 4128

作者：Ye, Jiang[1,2]; Zhu, Yunxia[2]; Hou, Bingbing[1,2]; Wu, Haizhen[1,2]; Zhang, Huizhan[1,2]

机构：[1] Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China; [2] Department of Applied Biology, East China University of Science and Technology, Shanghai, China

年份：2019

卷号：83

期号：3

起止页码：482

外文期刊名：Bioscience, Biotechnology and Biochemistry

收录：EI(收录号：20191006592738)

语种：英文

外文关键词：Antibiotics - Biochemistry - Bacteria - Genes

摘要：Bagremycin A and bagremycin B isolated from Streptomyces sp. Tü 4128 have activities against Gram-positive bacteria, fungi and also have a weak antitumor activity, which make them have great potential for development of novel antibiotics. Here, we report a draft genome 8,424,112 bp in length of S. sp. Tü 4128 by Illumina Hiseq2000, and identify the bagremycins biosynthetic gene cluster (BGC) by bioinformatics analysis. The putative bagremycins BGC includes 16 open reading frames (ORFs) with the functions of biosynthesis, resistance and regulation. Disruptions of relative genes and HPLC analysis of bagremycins production demonstrated that not all the genes within the BGC are responsible for the biosynthesis of bagremycins. In addition, the biosynthetic pathways of bagremycins are proposed for deeper inquiries into their intriguing biosynthetic mechanism. ? 2018 Informa UK Limited, trading as Taylor & Francis Group.