文献类型：期刊文献

中文题名：时间分辨荧光免疫分析仪测量时间稳定性的研究与设计

英文题名：Research and Design of Measuring Time Steady in Time-Resolved Fluoroimmunoassay Instrument

作者：王慧锋[1];潘德辉[2];张芹[3]

机构：[1]华东理工大学信息科学与工程学院,上海200237;[2]辽宁石油化工大学信息与控制工程学院,辽宁抚顺113001;[3]上海互帼科学仪器有限公司,上海200237

年份：2009

卷号：35

期号：2

起止页码：283

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：上海市重点学科建设项目(B504)

语种：中文

中文关键词：时间分辨荧光免疫分析仪;弱荧光信号测量;测量时间稳定性;游标法

外文关键词：time-resolved fluoroimmunoassay （TRFIA） instrument; weak fluorescence signal detection; stability of measuring time; vernier

摘要：时间分辨荧光免疫分析仪的测量精度取决于微弱荧光信号的测试精度,而测量时间不稳定将导致测量的荧光信号强度发生波动。针对影响测量时间精度的3个因素(时基信号误差、触发误差、量化误差),采用TX2108带温度补偿的晶体振荡器作为时基信号源,减小时基信号误差;采用RISC结构的高速单片机及性能稳定的74HC系列外围器件,减小触发误差;采用双游标法使测时分辨力提高3个数量级,减小量化误差。实验表明:在保证同样测量精度的前提下,所需测量次数由1 000次减少到120次。
The accuracy of time-resolved fluoroimmunoassay （TRFIA） instrument depends on that of the weak fluorescence signal detection, while the instability of the measuring time may result in that of intensity of fluorescence signal. Aiming at the three factors leading to the poor accuracy of the measuring time （the error in time basis, triggering error, quantization error）, TX2108 crystal oscillator with temperature compensating is employed as the time-basis signal source to reduce the error in time basis; RISC-base high speed MCU and high reliable 74HC chips are utilized to ensure the simultaneous performance of the optical excitation and fluorescence signals such that the triggering error can be reduced; Dual-vernier method is used to raise the measuring resolution for 3-times magnitude such that the quantization error is reduced. The experiment results show that, in the same measuring precision, measuring times are reduced from 1 000 to 120.