文献类型：期刊文献

英文题名：Insight into metabolism of CHO cells at low glucose concentration on the basis of the determination of intracellular metabolites

作者：Lu, Subiao[1]; Sun, Xiangming[1]; Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2005

卷号：40

期号：5

起止页码：1917

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：2005098855612);WOS:【SCI-EXPANDED(收录号:WOS:000227569000056)】；

语种：英文

外文关键词：CHO cells; low glucose; intracellular amino acids

摘要：Glucose pulse culture was employed to characterize the growth and metabolism of Chinese hamster ovary (CHO) cells at low glucose concentration. Cell growth was gradually limited when the glucose concentration decreased to below 1.22 mmol/l. Correspondingly, in comparison with the culture at above 4.8 mmol/l glucose, the intracellular concentrations of ATP decreased by 8%, 19% and 47% at the glucose concentrations of 1.2, 0.54, and 0.25 mmolA, respectively, showing the shortage of energy supply might result in growth limitation at low glucose concentration. The specific consumption rates of Gln, Ser, His, Thr, Arg, Tyr, Cys2, Val, Met, Phe, Ile, Len and Lys increased at glucose concentration below 0.54 mmol/l, implying that these amino acids were mainly catabolized instead of used as "building bricks" in protein synthesis under glucose limitation. Both intracellular Asp and Glu are always accumulated under any conditions, while intracellular concentrations of other amino acids, such as Ser, Thr, Arg, Tyr, Ala, Val, Met, Phe, Ile and Len, increased with the decrease in the glucose concentration. There was no obvious difference in the intracellular concentrations of organic acids at different glucose concentrations. Moreover, high concentrations of intracellular malate and undetectable iso-citrate were observed, implying that citrate might be intercepted from the TCA cycle for other anabolisms. (c) 2004 Elsevier Ltd. All rights reserved.