文献类型：期刊文献

英文题名：An interpenetrating network-strengthened and toughened hydrogel that supports cell-based nucleus pulposus regeneration

作者：Gan, Yibo[1];Li, Pei[1];Wang, Liyuan[1];Mo, Xiumei[2];Song, Lei[1];Xu, Yuan[3];Zhao, Chen[1];Ouyang, Bin[1];Tu, Bing[1];Luo, Lei[1];Zhu, Linyong[4];Dong, Shiwu[5];Li, Fuyou[6,7];Zhou, Qiang[1]

机构：[1]Third Mil Med Univ, Southwest Hosp, Dept Orthoped, Natl & Reg United Engn Lab Tissue Engn, Chongqing 400038, Peoples R China;[2]Donghua Univ, Coll Chem & Chem Engn & Biol Engn, Shanghai 201620, Peoples R China;[3]Third Mil Med Univ, Xinqiao Hosp, Dept Orthoped, Chongqing, Peoples R China;[4]East China Univ Sci & Technol, Inst Fine Chem, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[5]Third Mil Med Univ, Sch Biomed Engn, Dept Biomed Mat Sci, Chongqing 400038, Peoples R China;[6]Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China;[7]Fudan Univ, State Key Lab Mol Engn Polymers, Shanghai 200433, Peoples R China

年份：2017

卷号：136

起止页码：12

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20172003674190);WOS:【SCI-EXPANDED(收录号:WOS:000401816500002)】；

基金：This study was supported by the NSFC (grant number 81272029); the NSFC (grant number 81027005); the Transformation of Scientific and Technological Achievements of the Third Military Medical University (grant number 2011XZH006); and Key Project of Military Logistics (grant number BWS13C014-1). The authors thank Yujie Hua, Xiaohua Geng and Xi Wang for excellent technical support.

语种：英文

外文关键词：Intervertebral disc; Nucleus pulposus; Hydrogel; Interpenetrating network; Minimally invasive surgery; Tissue engineering

摘要：Hydrogel is a suitable scaffold for the nucleus pulposus (NP) regeneration. However, its unmatched mechanical properties lead to implant failure in late-stage disc degeneration because of structural failure and implant extrusion after long-term compression. In this study, we evaluated an interpenetrating network (IPN)-strengthened and toughened hydrogel for NP regeneration, using dextran and gelatin as the primary network while poly (ethylene glycol) as the secondary network. The aim of this study was to realize the NP regeneration using the hydrogel. To achieve this, we optimized its properties by adjusting the mass ratios of the secondary/primary networks and determining the best preparation conditions for NP regeneration in a series of biomechanical, cytocompatibility, tissue engineering, and in vivo study. We found the optimal formulation of the IPN hydrogel, at a secondary/primary network ratio of 1:4, exhibited high toughness (the compressive strain reached 86%). The encapsulated NP cells showed increasing proliferation, cell clustering and matrix deposition. Furthermore, the hydrogel could support long-term cell retention and survival in the rat IVDs. It facilitated rehydration and regeneration of porcine degenerative NPs. In conclusion, this study demonstrates the tough IPN hydrogel could be a promising candidate for functional disc regeneration in future. (C) 2017 Elsevier Ltd. All rights reserved.