文献类型：期刊文献

英文题名：A Monochromophoric Approach to Succinct Ratiometric Fluorescent Probes without Probe-Product Crosstalk

作者：Xin, Kai[1];Li, Xinxing[2];Guo, Yinghua[1];Zhong, Youhuan[3];Wang, Jungang[4];Yang, Haotian[5];Zhao, Jie[1];Guo, Chunlei[1];Huang, Yunxia[1];Lei, Zuhai[1];Ying, Yi-Lun[4];Luo, Xiao[1];Wang, Haolu[5];Qian, Xuhong[1];Yang, Wen[3];Liang, Xiaowen[2,5];Yang, Youjun[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Changzheng Hosp, Dept Gen Surg, Shanghai 200003, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Med, Dept Mol & Cellular Biochem, Shanghai 200025, Peoples R China;[4]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[5]Univ Queensland, Diamantina Inst, Woolloongabba, Qld 4102, Australia

年份：2021

卷号：3

期号：8

起止页码：2307

外文期刊名：CCS CHEMISTRY

收录：WOS:【ESCI(收录号:WOS:000794223800022)】；

基金：This work is supported by the National Natural Science Foundation of China (nos. 21822805, 21908065, 31871430, 81802979, 818800585, and 8180032537), Young Medical Talents Training Program of Shanghai (2018), the Commission of Science and Technology of Shanghai Municipality (no. 18430711000), the Australian National Health and Medical Research Council (nos. APP1126091 and APP1125794), the Science and Technology Commission of Shanghai Municipality for the Shanghai International Cooperation Program (18430711000), and the China Postdoctoral Science Foundation (nos. 2019M651427 and 2020T130197), W. Yang acknowledges the financial support of the innovative research team of high-level local universities in Shanghai.

语种：英文

外文关键词：ratiometric probe; single chromophoric; probe-product crosstalk; cellular imaging; total oxidative capacity

摘要：Ratiometric probes facilitate quantitative studies via self-calibration and are cherished for bioimaging. Often, a small probe-product spectral separation leads to crosstalk, but the rational development of ratiometric probes with zero probe-product crosstalk remains challenging. Harnessing the recent progress on photophysical modulation of xanthenoid fluorochromes, we propose a powerful and versatile probe design principle, that is, "bridging-group modification," and developed totalROX, a robust probe for monitoring the total cellular oxidative capacity. First, totalRox affirmatively detected the complete set of biorelevant highly oxidative species: per-acids (2 e(-)), radicals (1 e(-)), nitrosative (NO+) species, and singlet oxygen (O-1(2)). Nonoxidative or mildly oxidative pro-oxidants, for example, O-2(center dot-), H2O2, NO, ONOO-, and CIO- were not detected. Second, the absorption/fluorescence maxima of the probe (total-ROX, lambda(abs)/lambda(em) =425/525 nm) and the detection product (Ox670, lambda(abs)/lambda(em) = 650/675 nm) were separated by ca. 225/150 nm, respectively, which eliminated probe-product crosstalk. Third, it renders the ratio-metric signal with a single chromophore and is structurally succinct. TotalROX allowed quantitative analysis and was more sensitive than Amplex Red and CellROX Deep Red, two commercial probes for cell oxidative species. Bioimaging potentials of totalROX for monitoring cell redox status were exemplified in three different cell lines.