文献类型：期刊文献

英文题名：Interaction study of cancer cells and fibroblasts on a spatially confined oxygen gradient microfluidic chip to investigate the tumor microenvironment

作者：Sun, Wei[1];Chen, Yuqing[1];Wang, Yuerong[1];Luo, Pei[2];Zhang, Min[3,4];Zhang, Hongyang[1];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau Inst Appl Res Med & Hlth, Taipa, Macau, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Modern Engn Ctr TCM, Sch Pharm, Shanghai 200237, Peoples R China

年份：2018

卷号：143

期号：22

起止页码：5431

外文期刊名：ANALYST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000456684800012)】；

基金：This research was supported financially by the National Natural Science Foundation of China (No. 81573397). This is an open project of State Key Laboratory of Quality Research in Chinese Medicine (Macau University of Science and Technology, MUST-SKL-2016-06) funded by the Macao Science and Technology Development Fund, Macau Special Administrative Region.

语种：英文

摘要：This paper reports a single-layered microfluidic device for studying the interaction of cancer cells and fibroblasts in an oxygen gradient. This gradient can be established from 1.9% to 18.8% using a spatially confined oxygen scavenging chemical reaction. Due to the spatial design of the chip, only cancer cells can sustain low oxygen conditions when co-cultured with fibroblasts in the adjacent channels, simulating the cell-cell interactions of the hypoxic cancer cells and the surrounding fibroblasts in tumor microenvironment in vivo. Moreover, a cell migration assay is performed on the chip for studying the tumor invasion ability. The results show that the migration speed of B16 cells is increased by hypoxia and the co-culture with L929 cells. In addition, we use ELISA to quantify the migration-related cytokines transforming growth factor-beta 1 (TGF-beta 1) in the microfluidic system. Our results confirm interaction between cancer cells and fibroblasts. This microfluidic device provides new insight for the investigation of tumor microenvironment and cell interactions.