文献类型：期刊文献

英文题名：A polymer-based zwitterionic stationary phase for hydrophilic interaction chromatography

作者：Li, Shuxiang[1];Li, Zongying[1];Zhang, Feifang[1];Geng, Huiliang[1];Yang, Bingcheng[1]

机构：[1]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China

年份：2020

卷号：216

外文期刊名：TALANTA

收录：;EI(收录号：20201308353688);WOS:【SCI-EXPANDED(收录号:WOS:000536207600009)】；

基金：This work was sponsored by National Natural Science Foundation of China (No. 21974044) and National Key Research and Development Program of China (2018YFC1603500).

语种：英文

外文关键词：Hydrophilic interaction chromatography; Zwitterionic stationary phase; Polymer; Glycidyl methacrylate; Bleed

摘要：We describe a novel polymer-based zwitterionic stationary phase for hydrophilic interaction chromatography (HILIC). It is prepared by chemical modification of poly (glycidyl methacrylate-divinylbenzene) (GMA-DVB) by converting epoxide groups of microsphere surface to diol groups via hydrolysis, then clicking cysteine onto the microsphere with pendant double bonds of microsphere surface via "thiol-ene" click chemistry. The phase has been characterized by scanning electron micrograph, elemental analysis and zeta potential measurement. Diol and zwitterionic group (carboxylate and amine group associated with cysteine) onto the surface of GMA-DVB microspheres make them possess good hydrophilicity, as supported by effective separation towards common polar analytes. It shows good stability at alkaline solution (e.g. pH 10) and negligible bleed (e.g. only 1.7-fold blank and similar to 55-fold lower than a commercial silica-based polar phase tested with the minimal bleed level). Such phase exhibits specific separation selectivity to ionic analytes and simultaneous separation of anions and cations is achieved in the retention order of I- < NO3- < Choline < Br- < Cl- < K+ < Na+.