文献类型：期刊文献

英文题名：Damage of Escherichia coli membrane by bactericidal agent polyhexamethylene guanidine hydrochloride: micrographic evidences

作者：Zhou, Z. X.[3];Wei, D. F.[2];Guan, Y.[2];Zheng, A. N.[2];Zhong, J. J.[1,3]

机构：[1]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Minist Educ, Key Lab Microbial Metab, Shanghai 200240, Peoples R China;[2]E China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2010

卷号：108

期号：3

起止页码：898

外文期刊名：JOURNAL OF APPLIED MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000274389200016)】；

基金：The authors are grateful to the National Natural Science Foundation of China (no. 50573020) and the Shanghai Leading Academic Discipline Project (project nos. B203 and B505) for the financial support.

语种：英文

外文关键词：bactericidal mechanism; membrane; micrographic observation; polyhexamethylene guanidine hydrochloride

摘要：Aims: The purpose of this study was to provide micrographic evidences for the damaged membrane structure and intracellular structure change of Escherichia coli strain 8099, induced by polyhexamethylene guanidine hydrochloride (PHMG). Methods and Results: The bactericidal effect of PHMG on E. coli was investigated based on beta-galactosidase activity assay, fluorescein-5-isothiocyanate confocal laser scanning microscopy, field emission scanning electron microscopy and transmission electron microscopy. The results revealed that a low dose (13 mu g ml-1) of PHMG slightly damaged the outer membrane structure of the treated bacteria and increased the permeability of the cytoplasmic membrane, while no significant damage was observed to the morphological structure of the cells. A high dose (23 mu g ml-1) of PHMG collapsed the outer membrane structure, led to the formation of a local membrane pore across the membrane and badly damaged the internal structure of the cells. Subsequently, intracellular components were leaked followed by cell inactivation. Conclusions: Dose-dependent membrane disruption was the main bactericidal mechanism of PHMG. The formation of the local membrane pores was probable after exposure to a high dose (23 mu g ml-1) of PHMG. Micrographic evidences were provided about the damaged membrane structure and intracellular structure change of E. coli. Significance and Impact of the Study: The presented information helps understand the bactericidal mechanism of PHMG by membrane damage.