文献类型：期刊文献

英文题名：Transgenic eukaryotic microalgae as green factories: providing new ideas for the production of biologically active substances

作者：Shi, Qianwen[1,2];Chen, Cheng[2];Zhang, Wei[2];Wu, Ping[2];Sun, Meng[2];Wu, Hui[1];Wu, Haizhen[1,2];Fu, Pengcheng[3];Fan, Jianhua[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, 58 Renmin Ave, Haikou 570228, Hainan, Peoples R China

年份：2021

卷号：33

期号：2

起止页码：705

外文期刊名：JOURNAL OF APPLIED PHYCOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000613626600003)】；

基金：This work was sponsored by National Key Research and Development Project of China 2019YFA0906300 and 2020YFA0907304, Natural Science Foundation of Shandong Province ZR2019ZD17, National Natural Science Foundation of China 31872608, Natural Science Foundation of Shanghai 18ZR1410100, Funding Project of the State Key Laboratory of Bioreactor Engineering, and Open Funding Project of the State Key Laboratory of Marine Resource Utilization in South China Sea (Hainan University) 2019002.

语种：英文

外文关键词：Eukaryotic microalgae; Genetic engineering; Metabolism; Biopharmaceuticals production; Therapeutic proteins

摘要：Eukaryotic microalgae are important primary producers in nature that play an important role in the energy cycle of nature. The eukaryotic microalgae are also regarded as potential bioactive substance producers. In recent years, with the aid of genetic engineering, eukaryotic microalgae have found a wider range of potential applications in medicine, food, health products, cosmetics, and environmental protection. This article reviews the state of the art of microalga genetic engineering from the aspects of gene transfer system, gene editing technology, and screening method, with examples of the enhancement of lipids, carotenoids, polysaccharide, and functional proteins. Potential application scenarios of microalga genetic engineering and their products in the field of food and medicine are also highlighted. Furthermore, strategies for protein expression optimization in eukaryotic microalgae are reviewed. The existing shortcomings of eukaryotic microalga genetic engineering are also analyzed and highlighted.