文献类型：期刊文献

英文题名：Lithium hexamethyldisilazide initiated superfast ring opening polymerization of alpha-amino acid N-carboxyanhydrides

作者：Wu, Yueming[1];Zhang, Danfeng[1];Ma, Pengcheng[1];Zhou, Ruiyi[1];Hua, Lei[2];Liu, Runhui[1]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ,Res Ctr Biomed Mat, State Key Lab Bioreactor Engn,Key Lab Ultrafine M, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China

年份：2018

卷号：9

外文期刊名：NATURE COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000453057400003)】；

基金：This research was supported by the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), the National Natural Science Foundation of China (No. 21574038, 21774031), the National Key Research and Development Program of China (2016YFC1100401), the "Eastern Scholar Professorship" from Shanghai local government (TP2014034), the 1000 talent young scholar program in China, 111 project (B14018), the national special fund for State Key Laboratory of Bioreactor Engineering (2060204), the Fundamental Research Funds for the Central Universities (22221818014), the program for professor of special appointment at ECUST. The authors also thank Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization. The authors thank Professor Samuel H. Gellman for invaluable discussions on the manuscript.

语种：英文

摘要：Polypeptides have broad applications and can be prepared via ring-opening polymerization of alpha-amino acid N-carboxyanhydrides (NCAs). Conventional initiators, such as primary amines, give slow NCA polymerization, which requires multiple days to reach completion and can result in substantial side reactions, especially for very reactive NCAs. Moreover, current NCA polymerizations are very sensitive to moisture and must typically be conducted in a glove box. Here we show that lithium hexamethyldisilazide (LiHMDS) initiates an extremely rapid NCA polymerization process that is completed within minutes or hours and can be conducted in an open vessel. Polypeptides with variable chain length (DP = 20-1294) and narrow molecular weight distribution (Mw/Mn = 1.08-1.28) were readily prepared with this approach. Mechanistic studies support an anionic ring opening polymerization mechanism. This living NCA polymerization method allowed rapid synthesis of polypeptide libraries for high-throughput functional screening.