文献类型：期刊文献

英文题名：Real-time viable-cell mass monitoring in high-cell-density fed-batch glutathione fermentation by Saccharomyces cerevisiae T65 in industrial complex medium

作者：Xiong, Zhi-Qiang[1]; Guo, Mei-Jin[1]; Guo, Yuan-Xin[1]; Chu, Ju[1]; Zhuang, Ying-Ping[1]; Zhang, Si-Liang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, China

年份：2008

卷号：105

期号：4

起止页码：409

外文期刊名：Journal of Bioscience and Bioengineering

收录：EI(收录号：20082111269769)

语种：英文

外文关键词：Batch cell culture - Carbon dioxide - Fermentation - Yeast

摘要：An on-line monitoring of viable-cell mass in high-cell-density fed-batch cultivations of Saccharomyces cerevisiae grown on an industrial complex medium was performed with an in situ capacitance probe fitted to a 50-l fermentor. Conventional off-line biomass determinations of several parameters, including dry cell weight (DCW), optical density at 600 nm wavelength (OD600), packed mycelial volume (PMV) and number of colony forming units (CFU), were performed throughout the bioprocess and then compared with on-line viable-cell concentrations measured using a capacitance probe. Capacitance versus viable biomass and all off-line biomass assay values were compared during glutathione fermentation in industrial complex culture media. As a result, the relationship between the number of colony forming units and capacitance with a correlation coefficient (R) of 0.995 was achieved. Simultaneously, compared with those determined by at-line indirect estimation methods including oxygen uptake rate (OUR) and carbon dioxide evolution rate (CER), the specific growth rates estimated by on-line capacitance measurement could be more reliable during glutathione fermentation. Therefore, it is concluded that a capacitance probe is a practical tool for real-time viable biomass monitoring in high-cell-density fed-batch cultivation in a complex medium. ? 2008 The Society for Biotechnology, Japan.