文献类型：期刊文献

英文题名：In Situ Engineering Mesenchymal Stem Cells for Osteogenic Differentiation to Reverse Inflammaging and Restore Periodontal Bone Homeostasis

作者：Zhang, Fan[1,2];Tian, Yi[1];Fan, Zhen[1,3];Liu, Danqing[4];Dai, Ting[5];Guo, Qingyuan[6];Wang, Zhuo[1];You, Sulan[1];Yue, Guangna[1];Xia, Runpeng[1];Du, Jianzhong[1,7,8];Xu, Yubo[1,2]

机构：[1]Tongji Univ, Shanghai East Hosp, Sch Med, Dept Stomatol, Shanghai 200120, Peoples R China;[2]Jiangxi Prov Peoples Hosp, Dept Stomatol, Nanchang 330000, Jiangxi, Peoples R China;[3]Tongji Univ, Sch Mat Sci & Engn, Dept Polymer Mat, 4800 Caoan Rd, Shanghai 201804, Peoples R China;[4]Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Dept Orthoped, Shanghai 200072, Peoples R China;[5]Gen Hosp Northern Theater Command, Dept Stomatol, Shenyang 110016, Peoples R China;[6]Hlth Sci Univ Hokkaido, Sch Dent, Ishikari 0610293, Japan;[7]Tongji Univ, Sch Mat Sci & Engn, Minist Educ, Key Lab Adv Civil Engn Mat,Dept Polymer Mat, 4800 Caoan Rd, Shanghai 201804, Peoples R China;[8]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China

年份：2025

外文期刊名：ADVANCED FUNCTIONAL MATERIALS

收录：;EI(收录号：20253218963902);WOS:【SCI-EXPANDED(收录号:WOS:001546128100001)】；

基金：F.Z. and Y.T. contributed equally to this work. This work was supported by the Research Start-up Fund for Introduced Talent of Shanghai East Hospital (Grant No. DFRC2021006), National Natural Science Foundation of China (22335005, 52222306, 22475154), international scientific collaboration fund of Science and Technology Commission of Shanghai Municipality (23520710900), Innovation Program of Shanghai Municipal Education Commission (2023ZKZD28), and the Fundamental Research Funds for the Central Universities. The authors sincerely acknowledge Dr. Wenjun Le from Tongji University for his invaluable experimental support. The authors also extend the gratitude to Prof. Weitao Yang from the School of Medicine, Tongji University, for his insightful guidance and constructive discussions. Dr. Erik Jan Cornel helped review and revise the entire manuscript.

语种：英文

外文关键词：inflammaging; in situ engineering; mesenchymal stem cells; periodontal regeneration

摘要：Chronic inflammatory diseases such as periodontitis pose significant challenges for regenerative therapies, particularly stem cell-based approaches. Conventional ex vivo engineering strategies, in which nanoparticles or bioactive molecules are pre-loaded onto mesenchymal stem cells (MSCs) prior to transplantation, often disrupt cell phenotype, limit therapeutic efficacy, and result in poor retention and engraftment at target sites. In this study, an innovative in situ engineering strategy involving the co-delivery of MSCs with cerium-tannic acid@bovine serum albumin nanoparticles (CTB NPs) is introduced, which enables real-time osteogenic differentiation within the inflammatory microenvironment. Unlike traditional methods, this in situ approach harnesses the intrinsic homing and adaptive responses of MSCs within pathological niches, achieving real-time functional augmentation and precise biointerface modulation under inflammatory stress. This strategy increases MSC viability by 15%, enhances osteogenic differentiation by 70%, and reduces cellular senescence by 73.2%, as evidenced by decreased p21 expression. In a rat periodontitis model, CTB@MSCs markedly attenuate inflammation, reduce bone resorption by 56.25%, and restore alveolar bone height by 75%, demonstrating robust therapeutic potential in chronic inflammatory conditions. Collectively, this in situ MSC engineering platform offers a promising avenue for stem cell-based treatment of periodontitis and other inflammatory diseases.