文献类型：期刊文献

英文题名：Immobilization of penicillin G acylase on paramagnetic aldehyde-functionalized mesostructured cellular foams

作者：Yang, Ling[1];Gao, Zhenyuan[1];Guo, Yanglong[1];Zhan, Wangcheng[1];Guo, Yun[1];Wang, Yunsong[1];Lu, Guanzhong[1]

机构：[1]E China Univ Sci & Technol, Res Inst Ind Catalysis, Key Lab Adv Mat, Shanghai 200237, Peoples R China

年份：2014

卷号：60

起止页码：32

外文期刊名：ENZYME AND MICROBIAL TECHNOLOGY

收录：;EI(收录号：20142017715637);WOS:【SCI-EXPANDED(收录号:WOS:000337014300005)】；

基金：This project was supported by National Basic Research Program of China (2013CB933201, 2010CB732300), National Natural Science Foundation of China (21103048), Program for New Century Excellent Talents in University (NCET-09-0343), Shu Guang Project of Shanghai Municipal Education Commission and Shanghai Education Development Foundation (10SG30).

语种：英文

外文关键词：Mesostructured cellular foams; Paramagnetic; Aldehyde groups; Penicillin G acylase; Immobilization

摘要：Paramagnetic aldehyde-functionalized mesostructured cellular foams (PAMCFs), synthesized by grafting 3-aminopropyltriethoxysilane modified Fe3O4 (NH2-Fe3O4) nanoparticles with larger particle size than the window pore size of MCFs on the outer surface of aldehyde-functionalized mesostructured cellular foams (AMCFs), were investigated as efficient supports for immobilization of penicillin G acylase (PGA). The results show that NH2-Fe3O4 nanoparticles were successfully grafted on the outer surface of AMCFs and PGA molecules were mainly immobilized covalently on the inner surface of PAMCFs, which was because amino groups of NH2-Fe3O4 nanoparticles or PGA molecules reacted with aldehyde groups of AMCFs or PAMCFs to form imine bonds. PGA/PAMCFs-15 showed a rather high initial activity of 9563 U g(-1) and retained 89.1% of its initial activity after recycled for 10 times. PGA/PAMCFs are easily recycled by magnetic field in order to replace tedious separation of high-speed centrifugation for mesoporous materials. (C) 2014 Elsevier Inc. All rights reserved.