文献类型：期刊文献

中文题名：藻粉中叶黄素提取工艺的优化

英文题名：Optimization of Extraction Conditions of Lutein in Algae Powder

作者：盖霞[1];陆兵[1];夏杰[1];徐殿胜[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2015

卷号：41

期号：1

起止页码：43

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家重点基础研究发展计划项目(973计划)(2011CB200906)

语种：中文

中文关键词：酶提取法;藻粉;叶黄素;正交试验

外文关键词：enzyme extraction; algae powder; lutein; orthogonal test

摘要：采用酶提取法对藻粉中叶黄素提取工艺进行研究,实验分别考察了不同提取剂、不同种类酶、酶的使用量、酶解时间及搅拌时间对叶黄素提取的影响。在单因素实验的基础上,选取影响较大的3个因素采用L9(33)正交实验考察对叶黄素提取的影响,以叶黄素提取量为指标,确定了最佳工艺条件:酶液添加量为5mL,酶解时间2.5h,磁力搅拌时间2.5h,此条件下叶黄素最佳提取量为2.18mg/g。
The extraction process of lutein algae powder was studied by enzyme extraction,the affecting factors of lutein extraction such as different solvents,different kinds of enzyme,enzyme quantity,enzymolysis time and stirring time were investigated in single factor experiments.An orthogonal test table L9（33）was used to optimize the affecting factors on the base of single factor experiments.The content of lutein in the end product was determined.The results showed that the optimal extraction and saponification parameter are as follows,the enzyme liquid adding 5mL,hydrolysis time 2.5h,magnetic stirring time 2.5h.Under this condition,the yield of lutein reached 2.18mg/g.