文献类型：期刊文献

英文题名：Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column

作者：Wei, Jie[1];Shen, Aijin[2];Wan, Huihui[3];Yan, Jingyu[2];Yang, Bingcheng[1];Guo, Zhimou[2];Zhang, Feifang[1];Liang, Xinmiao[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China;[3]Dalian Univ Technol, Chem Anal & Res Ctr, Fac Chem Environm & Biol Sci & Technol, Dalian, Peoples R China

年份：2014

卷号：37

期号：14

起止页码：1781

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20143017983867);WOS:【SCI-EXPANDED(收录号:WOS:000339669700009)】；

基金：This work was financially supported by National Natural Science Foundation of China (no. 21177040, 21005077). We also thank the Open Foundation of Key Laboratory of Industrial Ecology and Environmental Engineering for financial support.

语种：英文

外文关键词：Aminoglycosides; Hydrophilic interaction chromatography; Separation selectivity; Zwitterionic stationary phases

摘要：Hydrophilic interaction liquid chromatography has emerged as a valuable alternative approach to ion-pair chromatography for the separation of aminoglycoside antibiotics in recent years. However, the resolution of structurally related aminoglycosides is a great challenge owing to the limited selectivity. In this work, a cysteine-based zwitterionic stationary phase (named Click TE-Cys) was utilized and compared with five commonly used hydrophilic interaction liquid chromatography columns. Click TE-Cys displayed much better selectivity for structurally similar aminoglycosides. The retention behaviors of aminoglycosides were investigated in detail, revealing that low pH (2.7 or 3.0) and high buffer concentration (>= 50 mM) were preferable for achieving good peak shape and selectivity. Effective resolution of ten aminoglycosides including spectinomycin, dihydrostreptomycin, streptomycin, gentamicin C1, gentamicin C2/C2a, gentamicin C1a, kanamycin, paromonycin, tobramycin, and neomycin was realized at optimized conditions. Additionally, spectinomycin and its related impurities were successfully resolved. The results indicated the great potential of the Click TE-Cys column in the separation of aminoglycoside mixtures and related impurities.