文献类型：期刊文献

英文题名：Regulated enzyme distribution for enhanced wearable sweat glucose monitoring

作者：Chu, Yao[1];Chen, Yangyang[1];Gu, Zhen[2];Liu, Xinyi[1];Cheng, Tao[1];Shen, Yichuan[1];Lv, Jian[3];Wu, Yiping[1];Chen, Nan[1];Yang, Haifeng[1];Tang, Wanxin[1]

机构：[1]Shanghai Normal Univ, Coll Chem & Mat Sci, Shanghai 200234, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Smart Mfg Energy Chem Proc, Minist Educ, Shanghai 200237, Peoples R China;[3]Xi An Jiao Tong Univ, Frontier Inst Sci & Technol FIST, Interdisciplinary Res Ctr Frontier Sci & Technol, Xian 710049, Shaanxi, Peoples R China

年份：2026

卷号：1382

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20254419430909);WOS:【SCI-EXPANDED(收录号:WOS:001610481100001)】；

基金：This project is supported by the National Natural Science Foundation (NSF) of China, grant number (22304118) , S & TCSM of Shanghai, grant number (23YF1431000) , Project of the National Key Research and Development Program of China, grant number (2023YFC3305802) , International Joint Laboratory on Resource Chemistry (IJLRC) , Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Engineering Research Center of Green Energy Chemical Engineering (18DZ2254200) , and the Shanghai Frontiers Science Center of Bio-mimetic Catalysis. The authors acknowledge Zhengzhong LvZeng from Shanghai Normal University for the fabrication of magnetic materials.

语种：英文

外文关键词：Sweat reservoir; Enzymatic regulation; Concentration gradient; Non-quasi-equilibrium; Signal distorted

摘要：As an electrochemical cell and sweat reservoir, hydrogel plays a significant role in wearable sweat glucose sensing. However, the uncontrolled and randomly distributed network in the hydrogel, the aggregation of enzyme and leaching from the sensing surface often cause a concentration gradient of sweat glucose, which could lead to the distorted signal. In this study, a magnetic porous hydrogel is designed with uniformly embedded GOx. Such hydrogel is integrated with MWCNTs, CaCO3, and glutaraldehyde-coated magnetic nanoparticles (G-Fe3O4 NPs). Acid etching of CaCO3 forms the porous structure of hydrogel with high water absorbency. G-Fe3O4 NPs provide controllable cross-linkers between GOx and hydrogel. Guided by an external magnet, GOx distribution in the hydrogel is oriented in a constant and uniform order, thus can avoid non-quasi-equilibrium and distorted glucose sensing that caused by GOx aggregation and leakage. Importantly, MG-MFPH-GOx hydrogel show enhanced sensitivity when tested for both on-body and finger sweat glucose sensing and the detection limit reaches 0.25 mu mol/L. Moreover, sweat glucose sensors based on MG-MFPH-GOx hydrogel show stable performance for 30 days. Therefore, the regulated enzymatic hydrogel prepared with the facility of an external magnetic field provides a new strategy for constructing wearable sensors.