文献类型：期刊文献

英文题名：A cell-free therapy for articular cartilage repair based on synergistic delivery of SDF-1 & KGN with HA injectable scaffold

作者：Wu, Huachu[1];Shen, Liang[1];Zhu, Zhu[2];Luo, Xusong[2];Zhai, Yishu[1];Hua, Xiaobin[1];Zhao, Shicheng[1];Cen, Lian[1];Zhang, Zhibing[3]

机构：[1]East China Univ Sci & Technol, Sch Chem Engn, Dept Prod Engn, Shanghai Key Lab Multiphase Mat Chem Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Dept Plast & Reconstruct Surg, Shanghai 200011, Peoples R China;[3]Univ Birmingham, Sch Chem Engn, Birmingham B15 2TT, W Midlands, England

年份：2020

卷号：393

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20201108289327);WOS:【SCI-EXPANDED(收录号:WOS:000543713600010)】；

基金：This study is financially supported by National "973" Project Foundation (2010CB944804) and Newton Research Collaboration award from The Royal Academy of Engineering.

语种：英文

外文关键词：Co-encapsulation; SDF-1; KGN; Cartilage regeneration; Injectable scaffold

摘要：This study was to co-encapsulate a chemokine (stromal cell-derived factor-1, SDF-1) and a chondroinductive molecule (kartogenin, KGN) within microspheres via microfluidics, and to incorporate them into a hyaluronic acid (HA) injectable scaffold for articular cartilage defect repair. HA injectable scaffold, as a cartilage-friendly microenvironment, was prepared by crosslinking HA with 1,4-butanediol diglycidyl ether. A microfluidic device was set up to prepare monodisperse PLGA microspheres (49 mu m) to load SDF-1 and KGN. An in vivo model of full-thickness articular cartilage defects in rabbits was applied to evaluate the reparative capacity of the current package. The SDF-1 and KGN were co-encapsulated simultaneously within the core and shell area of the microsphere with high loading efficiency and sustained release profiles of more than 2 months. The release profiles of them were highly matched and well fitted to a first-order mathematical model. These microspheres when incorporated into HA injectable scaffold were demonstrated to heal the full-thickness articular cartilage defects in rabbits. The regenerated tissue had the typical cartilage histological characters and integrated well with the surrounding tissue at 12w. This developed cell-free system could serve as an efficient therapy for articular cartilage defects treatment, serving as a supplementary way to cell based therapies.