文献类型：期刊文献

英文题名：Protective effects of the citrus flavanones to PC12 cells against cytotoxicity induced by hydrogen peroxide

作者：Lu, Yan-hua[1];Su, Ming-yuan[1];Huang, Hai-ya[1];Lin-Li[2];Yuan, Cai-gen[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Unilever R&D Shanghai, Shanghai, Peoples R China

年份：2010

卷号：484

期号：1

起止页码：6

外文期刊名：NEUROSCIENCE LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000282625600002)】；

基金：This work was supported by Shanghai-Unilever Research and Development Fund (09540715800) and partially supported by the National Natural Science Foundation of China (no. 30870253) and by Shanghai Leading Academic Discipline Project (B505), by the National Special Fund for State Key Laboratory of Bioreactor Engineering, grant no. 2060204.

语种：英文

外文关键词：Naringin; Nobiletin; PC12 cells; Oxidative stress; Hydrogen peroxide

摘要：Oxidative stress has been considered as a major cause of cellular injuries in a variety of clinical abnormalities. One of the plausible ways to prevent the reactive oxygen species (ROS)-mediated cellular injury is dietary or pharmaceutical augmentation of endogenous antioxidant defense capacity In this study, we investigated the protective actions of citrus flavanones naringin and nobiletin against the cytotoxicity induced by exposure to hydrogen peroxide (H2O2) (150 mu M, 3 h) in PC12 cells. The results showed that naringin and nobiletin inhibited the decrease of cell viability (MTT reduction), prevented membrane damage (LDH release), scavenged ROS formation, reduced caspase-3 activity, and attenuated the decrease of mitochondrial membrane potential (MMP), respectively, in H2O2-induced PC12 cells. Meanwhile, naringin and nobiletin increased superoxide dismutase (SOD) and glutathione (GSH) activity, while decreased malondialdehyde (MDA), the production of lipid peroxidation. in H2O2-induced PC12 cells In addition, the percentage of cells undergoing H2O2-induced apoptosis was decreased in the presence of naringin and nobiletin. These results first demonstrate that naringin and nobiletin, even at physiological concentrations, have neuroprotective effects against H2O2-induced cytotoxicity in PC12 cells. All the above results suggest that these dietary antioxidants are potential candidates for use in the intervention for neurodegenerative diseases (C) 2010 Elsevier Ireland Ltd All rights reserved