文献类型：期刊文献

中文题名：MDCK细胞微载体悬浮培养放大工艺研究

英文题名：A Study on Scale-up Process for Microcarrier Cultue of MDCK Cells Using Low Serum Medium

作者：龚迪[1];易小萍[1];张元兴[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2012

卷号：32

期号：9

起止页码：55

中文期刊名：中国生物工程杂志

外文期刊名：China Biotechnology

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：MDCK细胞;微载体;低血清培养基;消化放大

外文关键词：Madin-Darbin canine kidney （MDCK） cell;Microcarrier;Low serum medium;Scale up by digestion;

摘要：MDCK细胞对各种流感病毒具有高度敏感性,广泛应用于流感病毒的分离和疫苗制备。通过探索培养基中促进细胞贴壁的关键组分,并筛选水解物,开发了适合MDCK细胞生长的低血清培养基。发现钙、镁离子是细胞贴壁不可缺少的物质,麦麸水解物可以部分代替培养基中的血清。利用该低血清培养基,经过消化转移将MDCK细胞从5 L反应器放大至25 L反应器,微载体上细胞贴附均匀、生长旺盛,25 L反应器中培养48 h细胞密度可达30.5×105cells/ml。研究结果为工业规模反应器微载体悬浮培养MDCK细胞生产流感病毒奠定了基础。
Madin-Darby canine kidney （MDCK） cells have been widely used for influenza virus isolation and vaccine production since they are susceptible to infection with various influenza virus strains. To develop a low serum medium for MDCK cells, critical components that affect cell adhesion and favorable hydrolyzate were investigated and selected separately. Calcium and magnesium ions were found indispensable to cell adhesion, and serum could be partially replaced by wheat hydrolyzate in medium. MDCK cells were digested from microcarriers in 5 L bioreactor and then scaled up to 25 L bioreactor. The results showed that the low serum medium well supported the growth of MDCK cells with favorable cell distributions, and the viable cell density reached 30.5？105 cells/ml 48 h after inoculation in 25 L bioreactor. The work provided the necessary basis for large-scale production of avian influenza virus using microcarrier-based MDCK cell culture system.