文献类型：期刊文献

英文题名：MOFs-functionalized regenerable SERS sensor based on electrochemistry for pretreatment-free detection of serum alkaline phosphatase activity

作者：Xi, Cheng-Ye[1];Zhang, Min[1];Jiang, Lei[1];Chen, Hua-Ying[1];Lv, Jian[1];He, Yue[1];Hafez, Mahmoud Elsayed[1,2];Qian, Ruo-Can[1];Li, Da -Wei[1]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn, Key Lab Adv Mat,Shanghai Key Lab Funct Mat Chem,Jo, Shanghai 200237, Peoples R China;[2]Beni Suef Univ, Fac Sci, Dept Chem, Bani Suwayf 62511, Egypt

年份：2022

卷号：369

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20222712329291);WOS:【SCI-EXPANDED(收录号:WOS:000829232500007)】；

基金：Acknowledgements This work was financially supported by the National Natural Science Foundation of China (21974046, 22176058, 21977031) , Science and Technology Commission of Shanghai Municipality (19391901700, 19520744000, 19ZR1472300) , Postdoctoral Science Foundation of China (2021M691002, 2021M701195) , and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：SERS sensor; Alkaline phosphatase; MOFs; Pretreatment -free; Electrochemical regeneration

摘要：Alkaline phosphatase (ALP), widely distributing in the human body, associates with many liver and bone dis-eases. Herein, we designed a reusable surface-enhanced Raman scattering (SERS) sensor via electrochemical self -assembly of Au nanoparticles (AuNPs) and zinc-based metal organic frameworks (Zn-MOFs) at anodic aluminum oxide (AAO) membrane for detecting ALP activity. The detection is based on the sensitive SERS response of the newly synthesized 2-mercaptoquinone (2-MBQ) modified on AuNPs surface towards ascorbic acid (AA) formed from the dephosphorylation of ascorbic acid 2-phosphate (AA2P) in the presence of ALP. Benefitting from the sieving function of Zn-MOFs and superiority of SERS, the developed sensor exhibited strong anti-interference performance and wide detection range from 1 to 300 U/L with a detection limit of 0.38 U/L, which allowed the direct detection of ALP activity in serum without any pretreatment. Besides, we regenerated the used sensor through the electrochemical oxidation of the reduced probe molecule 2-mercaptohydroquinone (2-MHQ). The regenerated sensor exhibited good maintenance of sensitive response to ALP, in favor of improving the usage efficiency and cutting the test cost. This work provides a promising platform for early diagnosis of ALP-related diseases and paves the way towards developing regenerable and pretreatment-free SERS sensors.