文献类型：期刊文献

英文题名：Oxygen vectors used for S-adenosylmethionine production in recombinant Pichia pastoris with sorbitol as supplemental carbon source

作者：Zhang, Jian-Guo[1];Wang, Xue-Dong[1];Zhang, Ji-Ning[1];Wei, Dong-Zhi[1]

机构：[1]E China Univ Sci & Technol, Coll Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：105

期号：4

起止页码：335

外文期刊名：JOURNAL OF BIOSCIENCE AND BIOENGINEERING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000256857800005)】；

语种：英文

外文关键词：S-adenosylmethionine; fermentation; oxygen vectors; Pichia pastoris; supplemental carbon source

摘要：In order to increase the yield of S-adenosylmethionine (SAM) in recombinant Pichia pastoris, a strategy of adding oxygen vectors and supplemental carbon sources was described. Three organic solutions were used as oxygen vectors for SAM accumulation at different concentrations and addition times. Firstly, n-hexane (0.5%) or n-heptane (1.0%) was added after 72 It of cultivation to improve SAM production. Carbon metabolism was scarce during the induction phase because of low methanol concentration. Secondly, sorbitol (1.2%), selected from three candidates (glycerol, lactic acid, and sorbitol), was used as the supplemental carbon source. The yield of SAM was improved significantly (53.261/0) at 1.0% n-heptane added at 72 h (48 h induction), 1.2% sorbitol added at 72, 96, and 120 h of cultivation and 1.0% methanol added every 24 h during cultivation.