文献类型：期刊文献

英文题名：A new circular-shape microfluidic network for generating gradients of multiple substances -design, demonstration and application

作者：Chen, Yuqing[1];Sun, Wei[1];Luo, Pei[2];Zhang, Min[3,4];Wang, Yuerong[1];Zhang, Hongyang[1];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Macau Univ Sci & Technol, Macau Inst Appl Res Med & Hlth, State Key Lab Qual Res Chinese Med, Taipa, Macau, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Sch Pharm, Modern Engn Ctr TCM, Shanghai 200237, Peoples R China

年份：2019

卷号：283

起止页码：247

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20185006241262);WOS:【SCI-EXPANDED(收录号:WOS:000455854000030)】；

基金：This research was supported financially by the National Natural Science Foundation of China (No. 81573397). This is an open project of State Key Laboratory of Quality Research in Chinese Medicine (Macau University of Science and Technology, MUST-SKL-2016-06) funded by the Macao Science and Technology Development Fund, Macau Special Administrative Region.

语种：英文

外文关键词：Microfluidic chip; Gradient generator; Drug synergy

摘要：In this paper we aimed to figure out an effective platform for generating gradients of three or more substances and designed an original microfluidic network with circular shape controlling diffusive mixing of fluids. The generated gradients were calculated by a theoretical treatment using a CFD model and verified in the fluorescence experiments, certifying that they maintained a spatially and temporally stable linearity under the flow rate of 2 mu L/min. Finally, we applied the platform for generating gradients on study of the drug synergism. 5-fluorouracil and tanshinone IIA (Tan IIA) were used for biological applicability via stimulation of single or multidrug combination on A549 cells. Consistent with the traditional MTT assays, both of the two drugs inhibited the cancer cell growth effectively and the IC50 values of 5-fluorouracil and Tan IIA were 20.37 and 44.78 mg/L, while their combination was superior. This microfluidic network is a simple and reliable platform as a gradient generator for investigating the possible combination of multiple drug candidates and their optimum dosage.