文献类型：期刊文献

英文题名：Pattern analysis of conditional essentiality (PACE)-based heuristic identification of an in vivo colonization determinant as a novel target for the construction of a live attenuated vaccine against Edwardsiella piscicida

作者：Ma, Ruiqing[1];Yang, Guanhua[1];Xu, Rongjing[4];Liu, Xiaohong[1,3];Zhang, Yuanxing[1,3];Ma, Yue[1,3];Wang, Qiyao[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Shandong, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]Yantai Tianyuan Aquat Co Ltd, Yantai, Shandong, Peoples R China

年份：2019

卷号：90

起止页码：65

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000471085000007)】；

基金：This work was supported by grants from National Key Research and Development Program of China (2018YFD0900500 to QYW), National Natural Science Foundation of China (31602200 to XHL), the Ministry of Agriculture of China (CARS-47), and the Science and Technology Commission of Shandong and Shanghai Municipality (2017CXGC0103 and 17391902000).

语种：英文

外文关键词：Edwardsiella piscicida; In vivo colonization; Live attenuated vaccine; PACE

摘要：Edwardsiella piscicida is the aetiological agent of fish edwardsiellosis, causing huge economic losses in aquaculture industries. The use of a live attenuated vaccine (LAV) will be an effective strategy to control the disease in farmed fish. Thus, methods facilitating exploration of targets used for construction of an LAV will be of great significance. Previously, we devised an algorithm termed pattern analysis of conditional essentiality (PACE) to perform genome-wide analysis of the temporal dynamic behaviour of E. piscicida mutants colonizing turbot. Here, we correlated the conditional essentiality patterns of the PACE-derived colonization determinants with that of the aroC gene encoding chorismate synthase, the established target for LAV construction in E. piscicida, and identified ETAE_0023 as a novel valuable LAV target. ETAE_0023 encodes an uncharacterized DcrB family protein. Deletion of ETAE_0023 dramatically impaired E. piscicida invasion capability in ZF4 cells as well as colonization in fish and resulted in in vivo clearance at similar to 30 days post-infection. Delta ETAE_0023 showed an similar to 2500-fold higher 50% lethal dose (LD50) than that of the wild type strain. Vaccination with Delta ETAE_0023 by intraperitoneal (i.p.) injection upregulated expression of immune factors, i.e., IL-1 beta, IgM, MHC-I and MHC-II, and produced significantly high levels of E. piscicida-specific IgM as well as serum bactericidal capacities in turbot. Moreover, a single i.p. inoculation with Delta ETAE_0023 generated significant protection comparable to the established WED LAV strain in turbot against challenge with the wild type strain after 5 weeks of vaccination. Taken together, we demonstrated a PACE-based method for heuristic identification of targets for LAV construction and presented Delta ETAE_0023 as a new LAV candidate against edwardsiellosis.