文献类型：期刊文献

中文题名：仿DNase酶的双金属分级多孔MOFs:制备与抗细菌生物膜应用

英文题名：DNase-mimetics based on bimetallic hierarchically macroporous MOFs for the efficient inhibition of bacterial biofilm

作者：夏凡[1];李可[1];杨健[1];陈婧雯[1];刘熙濛[1];龚鸣[1];顾金楼[1]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2024

卷号：67

期号：1

起止页码：343

中文期刊名：Science China Materials

外文期刊名：中国科学（材料科学）（英文版）

收录：CSTPCD;;EI(收录号：20240115318194);Scopus;WOS:【SCI-EXPANDED(收录号:WOS:001134652700003)】；CSCD:【CSCD2023_2024】；PubMed;

基金：This work was financially supported by the National Natural Science Foundation of China (22275054, 52103314, 21975072, and 51902106), Chenguang Plan of Shanghai Education Development Foundation (21CGA38), and the Program of Shanghai Academic/Technology Research Leader (23XD1401000).

语种：中文

中文关键词：金属有机框架;细菌生物膜;双金属;医疗器械;水解速率;细菌粘附;PVDF膜;大孔结构;

外文关键词：hierarchically porous MOFs;macro-microporous UiO-66;DNase;bacteria;biofilms

摘要：本研究成功构建了具有切割DNA能力的双金属枝状大孔金属有机框架(MOFs;HMUiO-66(Zr/Ce)),并将其用于高效破坏生物膜并抑制细菌生长.通过改变Zr/Ce的投料比,可在0-69%的范围内精确调控Zr/Ce的引入量,并控制其粒径在1μm-150 nm之间.HMUiO-66(Zr/Ce)具有独特的化学和热稳定性,开放的大孔结构和可接近的Lewis酸活性位点,表现出模拟DNA酶活性.双金属MOFs中丰富的Zr-OH位点能有效地捕获核酸,而相邻的Ce-OH基团对磷氧键形成亲核攻击,协同放大DNA的水解速率,使得开发的HMUiO-66(Zr/Ce)能够作为切割细胞外DNA和清除细菌生物膜的纳米药物.在此基础上,我们设计了一种仿生HMUiO-66(Zr/Ce)/PVDF膜,该生物膜可抑制细菌粘附和定植,在抗菌治疗和医疗器械中具有广阔的应用前景.
The accumulation of pathogenic biofilms poses a serious threat to human health,making their effective de-struction and eradication significant,yet greatly challenging.Herein,the bimetallic hierarchically macroporous metal-or-ganic frameworks(MOFs)(HMUiO-66(Zr/Ce))with ability to cleave DNA were successfully constructed for the efficient destruction of biofilm and thus inhibition of bacterial growth.By systematically adjusting the feed ratios of Zr/Ce,their particle size could be minimized to approximately 150 nm and the amount of Zr introduced into HMUiO-66(Zr/Ce)could be precisely tailored over a broad molar range from 0 to 69%.The developed HMUiO-66(Zr/Ce)feature unique chemical and thermal stabilities as well as abundant exposed Lewis acid sites.Benefitting from their open macroporous structure and accessible active sites,they exhibit exceptional DNase-mimetic activities.The abundant Zr-OH sites present in bimetallic MOFs could effectively sequester nucleic acids,while adjacent Ce-OH moieties form nucleophilic attacks toward phos-phorus-oxygen bonds,synergistically amplifying the hydro-lysis rate of DNA.Such a unique DNA cleavage ability makes the developed HMUiO-66(Zr/Ce)competent to serve as na-nomedicines for cleaving cross-linked extracellular DNA and eradicating bacterial biofilms.On this basis,we designed a biomimetic HMUiO-66(Zr/Ce)/polyvinylidene difluoride(PVDF)film which could discernibly suppress bacterial ad-herence and colonization,prefiguring their broad application potentials in antimicrobial therapy and medical devices.