文献类型：期刊文献

英文题名：Characterization of an exopolysaccharide synthesized by Lacticaseibacillus rhamnosus B6 and its immunomodulatory activity in vitro

作者：Han, Jin[1];Xia, Wei[2];Wang, Danqi[1];Wang, Yitian[1];Liu, Zhenmin[1];Wu, Zhengjun[1]

机构：[1]Bright Dairy & Food Co Ltd, Dairy Res Inst, State Key Lab Dairy Biotechnol, Shanghai Engn Res Ctr Dairy Biotechnol, Shanghai 200436, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China

年份：2024

卷号：264

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20241115714069);WOS:【SCI-EXPANDED(收录号:WOS:001209667800001)】；

基金：This study was supported by The 14th five-year National Key Research and Development Plan (2022YFD2100704) of China and Municipal State-owned Assets Supervision and Administration Commission enterprise innovation development and energy upgrading project (2022013) , Shanghai, China.

语种：英文

外文关键词：Lacticaseibacillus rhamnosus B6; Exopolysaccharide; Purification; Structure elucidation; Immunomodulatory activity

摘要：An exopolysaccharide, designated F1, was purified from the fermented milk by Lacticaseibacillus rhamnosus strain B6 (CGMCC No. 13310). F1, with the weight average molecular weight of 1.577 x 106 Da, is consisted of rhamnose, glucose and galactose in a molar ratio of 3.7:1.5: 1. The backbone included 1,3-linked Rha, 1,2,3linked Rha, 1,2-linked Glc and 1,3-linked Glc residues, with the branching point located at O2 position of 1,2,3-linked Rha residue, and the branch chain composed of terminal linked galactose residue with a pyruvate substituent. F1 could significantly stimulate the phagocytic activity and TNF-alpha expression in RAW 264.7 macrophages in a dose-dependent manner, and the release of NO at 200 mu g/mL as well. F1 at 200 mu g/mL could stimulate the expression of the pro-inflammatory cytokine encoding genes including TNF-alpha and iNOS, but with a negligible upregulating effect on the mRNA expression of IL-10. F1 could up-regulate the expression of NF-kappa Bp65 and skew macrophage polarization towards M1 phenotype. These results suggest F1 elicit an immunomodulatory effect through the NF-kappa B signaling pathway.