文献类型：期刊文献

英文题名：Sulfated Polysaccharide Regulates the Homing of HSPCs in a BMP-2-Triggered In Vivo Osteo-Organoid

作者：Dai, Kai[1];Zhang, Wenchao[1];Deng, Shunshu[1];Wang, Jing[1];Liu, Changsheng[1]

机构：[1]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2023

卷号：10

期号：24

外文期刊名：ADVANCED SCIENCE

收录：;EI(收录号：20232614304168);WOS:【SCI-EXPANDED(收录号:WOS:001016598900001)】；

基金：The authors thank the staff members of the Integrated Laser Microscopy System and Animal Facility at the National Facility for Protein Science in Shanghai (NFPS), Shanghai Advanced Research Institute, Chinese Academy of Sciences, Zhangjiang Lab, China, as well as Shanghai Jiao Tong University School of Medicine (SHSMU) Flow Cytometry Core for sample preparation, data collection and analysis. The authors also thank Y. Yu at ECUST for the help in polysaccharide synthesis, as well as T. Shen at ECUST for the help in schematic design. This research was supported by the Basic Science Center Program (No. T2288102), the Key Program of the National Natural Science Foundation of China (No. 32230059), the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), the National Key Research and Development Program of China (No. 2018YFE0201500), the National Natural Science Foundation of China (No. 31870953), the Foundation of Frontiers Science Center for Materiobiology and Dynamic Chemistry (No. JKVD1211002), the Wego Project of Chinese Academy of Sciences (No. (2020) 005), the Project of National Facility for Translational Medicine (Shanghai) (No. TMSK-2021-134), and the China Postdoctoral Science Foundation (No. 2022M721147).

语种：英文

外文关键词：hematopoietic stem cell transplantation; hematopoietic stem; progenitor cells; homing; in vivo osteo-organoid; sulfated polysaccharide

摘要：Hematopoietic stem cell transplantation (HSCT) is a well-established method for a variety of acquired and congenital diseases. However, the limited number and sources of therapeutic hematopoietic stem/progenitor cells (HSPCs) hinder the further application of HSCT. A BMP-2 triggered in vivo osteo-organoid that is previously reported, serves as a kind of stem cell biogenerator, for obtaining therapeutic HSPCs via activating the residual regenerative capacity of mammals using bioactive biomaterials. Here, it is demonstrated that targeting the homing signaling of HSPCs elevates the proportions and biological functions of HSPCs in the in vivo osteo-organoid. Notably, it is identified that sulfonated chito-oligosaccharide, a degradation product of sulfonated chitosan, specifically elevates the expression of endothelial protein C receptor on HSPCs and vascular cell adhesion molecule-1 on macrophages in the in vivo osteo-organoid, ultimately leading to the production of adequate therapeutic HSPCs. This in vivo osteo-organoid approach has the potential to provide an alternative HSPCs source for HSCT and benefits more patients.