文献类型：期刊文献

英文题名：Alcohol Dehydrogenase-Catalyzed Gold Nanoparticle Seed-Mediated Growth Allows Reliable Detection of Disease Biomarkers with the Naked Eye

作者：Peng, Mao-Pan;Ma, Wei[1];Long, Yi-Tao

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2015

卷号：87

期号：12

起止页码：5891

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20152500962474);WOS:【SCI-EXPANDED(收录号:WOS:000356755100008)】；

基金：This research was supported by the 973 Program (Grants 2013CB733700 and 2014CB748500), National Natural Science Foundation of China (Grants 21125522 and 21305045), Fundamental Research Funds for the Central Universities (Grant 222201313004) and Postdoctoral Science Special Foundation of China (Grant 2014T70398).

语种：英文

外文关键词：Plasmonics - Biomarkers - Catalysis - Eye protection - Metal nanoparticles

摘要：Here, we reported a strategy-based plasmonic enzyme-linked immunosorbent assay (ELISA) using alcohol dehydrogenase-catalyzed gold nanoparticle seed-mediated growth to serve as a colorimetric signal generation method for detecting disease biomarkers with the naked eye. This system possesses the advantages of outstanding robustness, sensitivity, and universality. By using this strategy, we investigated the hepatitis B surface antigen (HBsAg) and alpha-fetoprotein (AFP) with the lowest concentration of naked-eye detection down to 1.0 X 10(-12) g mL(-1). Experiments with real serum samples from HBsAg-infected patients are presented, demonstrating the potential for clinical analysis. Our method eliminates the need for sophisticated instruments and high detection expenses, making it possible to be a reliable alternative in resource-constrained regions.