文献类型：期刊文献

英文题名：Heterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stover

作者：Yi, Xia[1,2];Gao, Qiuqiang[1];Zhang, Lei[3];Wang, Xia[1,4];He, Yanqing[1];Hu, Fengxian[1];Zhang, Jian[1];Zou, Gen[3];Yang, Shihui[4];Zhou, Zhihua[3];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiujiang Univ, Jiangxi Prov Lab Syst Biomed, 17 Lufeng Rd, Jiujiang 332000, Peoples R China;[3]Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, CAS Key Lab Synthet Biol, Shanghai 200032, Peoples R China;[4]Hubei Univ, Coll Life Sci, Hubei Key Lab Ind Biotechnol, Wuhan 430062, Hubei, Peoples R China

年份：2019

卷号：12

期号：1

外文期刊名：BIOTECHNOLOGY FOR BIOFUELS

收录：;EI(收录号：20192507064144);WOS:【SCI-EXPANDED(收录号:WOS:000468763500002)】；

基金：This research was partially supported by the Natural Science Foundation of China (31961133006), the National Key Research and Development Program of China (2017YFB0309302), the Shanghai Pujiang Program (18PJD013), and the National Basic Research Program of China (2011CB707406).

语种：英文

外文关键词：Biodetoxification; Amorphotheca resinae ZN1; Heterozygous diploid; Gene pair; Coordinate expression

摘要：Background: Fast, complete, and ultimate removal of inhibitory compounds derived from lignocellulose pretreatment is the prerequisite for efficient production of cellulosic ethanol and biochemicals. Biodetoxification is the most promising method for inhibitor removal by its unique advantages. The biodetoxification mechanisms of a unique diploid fungus responsible for highly efficient biodetoxification in solid-state culture was extensively investigated in the aspects of cellular structure, genome sequencing, transcriptome analysis, and practical biodetoxification. Results: The inborn heterozygous diploid structure of A. resinae ZN1 uniquely contributed to the enhancement of inhibitor tolerance and conversion. The co-expression of gene pairs contributed to the enhancement of the degradation of lignocellulose-derived model inhibitors. The ultimate inhibitors degradation pathways and sugar conservation were elucidated by microbial degradation experimentation as well as the genomic and transcriptomic sequencing analysis. Conclusions: The finding of the heterozygous diploid structure in A. resinae ZN1 on biodetoxification took the first insight into the global overview of biodetoxification mechanism of lignocellulose-derived inhibitors. This study provided a unique and practical biodetoxification biocatalyst of inhibitor compounds for lignocellulose biorefinery processing, as well as the synthetic biology tools on biodetoxification of biorefinery fermenting strains.