文献类型：期刊文献

英文题名：Biosynthesis of α-Pinene by Genetically Engineered Yarrowia lipolytica from Low-Cost Renewable Feedstocks

作者：Wei, Liu-Jing[1];Zhong, Yu-Tao[1];Nie, Ming-Yue[1];Liu, Shun-Cheng[1];Hua, Qiang[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2021

卷号：69

期号：1

起止页码：275

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20210209763129);WOS:【SCI-EXPANDED(收录号:WOS:000611076800026)】；

基金：We thank Prof. Jie Bao for the generous gift of detoxified lignocellulosic hydrolysate. This research was financially supported by the National Key R&D Program of China (2019YFA0904302), National Natural Science Foundation of China (21776081), and the Natural Science Foundation of Shanghai (18ZR1410000).

语种：英文

外文关键词：Yarrowia lipolytica; alpha-pinene; waste cooking oil; lignocellulosic hydrolysate

摘要：alpha-Pinene, an important biologically active natural monoterpene, has been widely used in fragrances, medicines, and fine chemicals, especially, in high-density renewable fuels such as jet fuel. The development of an alpha-pinene production platform in a highly modifiable microbe from renewable substitute feedstocks could lead to a green, economical avenue, and sustainable biotechnological process for the biosynthesis of alpha-pinene. Here, we report engineering of an orthogonal biosynthetic pathway for efficient production of alpha-pinene in oleaginous yeast Yarrowia lipolytica that resulted in an alpha-pinene titer of 19.6 mg/L when using glucose as the sole carbon source, a significant 218-fold improvement than the initial titer. In addition, the potential of using waste cooking oil and lignocellulosic hydrolysate as carbon sources for alpha-pinene production from the engineered Y. lipolytica strains was analyzed. The results indicated that alpha-pinene titers of 33.8 and 36.1 mg/L were successfully obtained in waste cooking oil and lignocellulosic hydrolysate medium, thereby representing the highest titer reported to date in yeast. To our knowledge, this is also the first report related to microbial production of alpha-pinene from waste cooking oil and lignocellulosic hydrolysate.