文献类型：期刊文献

中文题名：造血细胞体外悬浮培养和生物反应器开发

英文题名：In vitro Suspension and Bioreactor Culture of Hematopoietic Cells

作者：迟占有[1];夏泉鸣[1];康自珍[1];谭文松[1];戴干策[1]

机构：[1]华东理工大学生物反应器国家重点实验室,上海200237

年份：2003

卷号：19

期号：5

起止页码：587

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：MEDLINE(收录号：15969089);CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家"8 63"计划生物领域项目;上海市现代生物与医药项目基金资助 (No.0 0 43 190 0 3 )~~

语种：中文

中文关键词：造血细胞;悬浮培养;生物反应器;培养环境

外文关键词：hematopoietic cell, stirred culture, bioreactor

摘要：为解决造血细胞的静态培养中由浓度梯度引起的培养不稳定、环境不均一、难放大等问题 ,首先采用转瓶对脐血单个核细胞进行了悬浮培养研究 ,结果表明 ,悬浮培养中总细胞、集落和CD34+ 细胞的扩增都高于静态的方瓶培养。在测试了所用材料生物相容性的基础上 ,开发了可以控制溶氧和pH的生物反应器 ,并将其应用到造血细胞的批培养中 ,结果表明反应器的培养环境均一 ,可实现较高密度的培养 ,而且总细胞、集落和CD34+ 细胞的扩增都优于静态培养。大规模的反应器培养有利于解决临床应用中细胞数量不足的问题。
Stirred culture offers a number of advantages over static systems as it maintains a stable, homogeneous culture environment and is easy to scale up. This paper focused on the development and application of stirred tank bioreactor to culture hematopoietic cells. Preliminary study of stirred culture of hematopoietic cells was carried out in cord blood mononuclear cells culture in spinner flask. The results showed that the amplification rates of total cell, CFU GM and BFU E, with the exception of CFU Mk, were greater in spinner flask than T flask. The number of total cells increased 20 fold after 14 days incubation in spinner flask. The amplification rates of CFU GM, CFU Mk and BFU E reached maximum at 10 th day, 10 th day and 7 th day respectively, and the maximal amplification rates were 9.2 fold, 5.5 fold and 2.4 fold respectively, whereas the rate of CD34 + cells in spinner flask was(6.7±4.0) fold at day 10. These results indicated that the stirred culture system is better than the static culture systems for hematopoietic cell proliferation. The biocompatibility of cord blood MNC to different types of materials used in bioreactors was also tested. The results showed that glass, stainless steel 316L and polytetraflouroethylene (PTFE) supported the growth of hematopoietic cells well. A higher cell density was reached in stirred bioreactors with controlled pH and DO than static culture. These findings suggested that the controlled large scale culture could be used to overcome the clinical shortage of hematopoietic cells.